响应面法优化葛根总黄酮的超声辅助提取工艺

为提高葛根总黄酮的得率，采用响应面试验设计优化超声辅助提取葛根总黄酮的工艺条件。在单因素试验基础上确定以超声时间、料液比及超声温度为3个主要因素，以总黄酮得率为响应值，根据Box-Behnken原理采用三因素三水平响应面试验设计进行优化。结果表明超声辅助法提取葛根总黄酮的最佳工艺条件为：以蒸馏水作为超声提取溶剂，超声时间43min、料液比1:10(g/mL)、超声温度68℃，总黄酮得率为5.28%，与预测得率5.36%相比，相对误差仅为1.49%。说明该模型可靠，与实际情况拟合较好。

Response Surface Methodology for Optimization of Ultrasound-Assisted Extraction of Flavonoids from Radix Puerariae

Response surface methodology was employed to optimize the ultrasound-assisted extraction of flavonoids from Radix Puerariae.The effects of extraction time,solid-to-liquid ratio,and temperature on the extraction yield of flavonoids were studied by one-factor-at-a-time method.A regression model for the yield of flavonoids was established using a Box-Behnken design involving the above three factors at three levels.The results showed that the optimal extraction conditions were as follows: distilled water as extraction solvent,extraction time of 43 min,solid-to-liquid ratio of 1:10(g/mL),and temperature of 68 ℃.Under these conditions,the observed yield of flavonoids was 5.28% in comparison with the predicted yield of 5.36%,and the relative error was only 1.49%,suggesting that the optimal extraction conditions are reliable.