饮料中胭脂红的荧光测定方法研究

建立了荧光分光光度法检测定饮料中的合成色素胭脂红的新方法。以320.0 nm为激发波长时,胭脂红有一灵敏的荧光发射现象,最大发射波长为432.0 nm。在2.00×10-7～2.20×10-5mol/L浓度范围内,胭脂红的荧光强度与浓度呈良好的线性关系,线性回归方程为F(%)=2.93×106c(mol/L)+12.9(相关系数r=0.994),检出限为1.00×10-7mol/L。将该方法应用于两种市售饮料中胭脂红的测定,加标回收率在94.4%～109.2%之间。

Fluorescence Spectrophotometry Analysis of Carmine in Drinks

A new fluorometric spectrophotometry for the determination of synthetic food coloring carmine was estab- lished. Carmine had sensitive fluorescence efficiency with max emission wavelength in 432.0 nm under 320.0 nm excita- tion fluorescence. A good linearity was obtained in the range of 2.00 × 10 -7 - 2.20 × 10 -5 mol/L, and its linear regres- sion equation was F（% ） = 2.93 × 106c（mol/L） ＋ 12.9（r =0.994）. The method was used to detect the content of car- mine in two drinks, and the recovery was 94.4% - 109.2%.