胶原纤维吸附剂对芹菜素的分离纯化性能研究

以戊二醛交联胶原纤维为吸附剂,通过柱层析研究其对芹菜素、槲皮素和杨梅素组成的三元黄酮类混合物中芹菜素的分离纯化特性。结果表明胶原纤维吸附剂对3种黄酮化合物表现出一定的吸附差异,对芹菜素的吸附作用最小;用100%～70%乙醇水溶液分步淋洗,能够从混合物中分离得到芹菜素,其纯度为98.03%,回收率为100%;用40%～60%乙醇水溶液分步淋洗,能够分离得到芹菜素和槲皮素,产物纯度分别为99.16%和99.47%,回收率分别为100%和91.22%;适当增大吸附柱的柱高,有助于提高芹菜素的纯度;在5次重复分离实验中,胶原纤维吸附剂的分离性能无明显降低,具有较好的重复使用性。因此,胶原纤维吸附剂可用于黄酮类混合物中芹菜素的分离纯化。

The study on separation and purification of apigenin using collagen fiber adsorbent

Collagen fiber,cross-linked by glutaraldehyde,was used as an adsorbent for the chromatographic separation and purification of apigenin from the mixture of flavonoids of apigenin,quercetin and myricetin.It was found that the collagen fiber adsorbent shows difference on adsorption ability to apigenin,quercetin and myricetin.The adsorption capacity of apigenin is relatively lowest.Apigenin could be well separated from the mixture by stepwise elution using 100%-70% aqueous ethanol solutions,and its purity was 98.03% and the recovery extent was 100%.While the apigenin and quercetin could be separated from the mixture system by stepwise elution using 40%-60% aqueous ethanol solutions,and their purity was 99.16% and 99.47%,and the recovery extent was 100% and 91.22%,respectively.It was also found that increasing the column heignt is helpful to improve the purity of apigenin.In addition,the separation capability of collagen fiber adsorbent was not obviously reduced after 5 times of repeated applications,exhibiting good reusability.Therefore,the collagen fiber adsorbent can be used for the effective separation and purification of apigenin from the mixture of flavonoids.