| NaNO2-Al（NO3）3显色分光光度法测定萼翅藤叶总黄酮的含量 |
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| 引用本文： | 王晓军,刘佳佳,李霞,任娜. NaNO2-Al（NO3）3显色分光光度法测定萼翅藤叶总黄酮的含量[J]. 广东化工, 2008, 35(11) |
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| 作者姓名： | 王晓军 刘佳佳 李霞 任娜 |
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| 作者单位： | 中南大学化学化工学院医药生物化工研究所 |
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| 摘 要： | 采用NaNO2-Al(NO3)3显色分光光度法测定萼翅藤叶提取液中总黄酮含量,并对测定条件进行研究。结果表明:以芦丁为标样,以NaNO2-Al(NO3)3为显色剂,碱性条件下在508 nm处测定显色液的吸光度确定总黄酮的含量,回归方程A=0.523C-0.0122,相关系数R=0.9998,方法的回收率为90.28%～99.73%,相对标准偏差(RSD)为1.57%;本方法简便、快速。
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| 关 键 词： | 萼翅藤 总黄酮 NaNO2-Al(NO3)3 分光光度法 |
NaNO_2-Al(NO_3)_3 Spectrophotometric Determination of Total Flavonoids in Calycopteris Floribunda Leaves |
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| Wang Xiaojun,Liu Jiajia,Li Xia,Ren Na. NaNO_2-Al(NO_3)_3 Spectrophotometric Determination of Total Flavonoids in Calycopteris Floribunda Leaves[J]. Guangdong Chemical Industry, 2008, 35(11) |
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| Authors: | Wang Xiaojun Liu Jiajia Li Xia Ren Na |
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| Abstract: | A method for the NaNO2-Al(NO3)3 spectrophotometric determination of total flavonoids in Calycopteris floribunda leaves was described.The conditions of determination were studied.Results showed: Rutin as standard sample,NaNO2-Al(NO3)3 was used as a chromogenic agent,on condition of basic property,total flavonoids was determined by spectrophotometer at 508 nm.Regression equation was A=0.523C-0.0122,correlation coefficient R=0.9998,The RSD was 1.57 %.The method was simple and rapid. |
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| Keywords: | calycopteris floribunda leaves total flavonoids NaNO2-Al(NO3)3 spectrophotometry |
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