双酶水解法制备羊胎盘活性肽

采用现代生物酶解技术制备羊胎盘生物活性肽 ,以水解度 (DH)和酸溶性肽得率(YASP)为指标 ,在单酶水解的基础上探索更加有效的新工艺———双酶水解工艺。实验确定了木瓜蛋白酶 (Papain)与胰蛋白酶 (Trypsin)的等活力混合酶为水解羊胎盘的最佳双酶组合 ,其最佳酶解工艺条件为 :pH7 0、温度 5 2℃、酶活添加量 3 0 0 0u/ g(料 )、底物浓度 10 %、水解时间 3h ,酶解前最佳热处理条件为沸水浴 15min。

Preparation of Active Peptide from Sheep Placenta by Bienzyme Hydrolysis

Bioactive peptide was prepared by enzymatic hydrolysis of proteins from sheep placenta. By measuring the degree of hydrolysis and the yield of acid-soluble peptide, bienzyme hydrolysis was studied on the basis of the experimental results of mono-enzyme hydrolysis. The mixture of Papain and Trypsin with the same activities was selected as the optimum hydrolytic condition,and other optimum parameters of the bienzyme hydrolysis were:the pH was 7.0,the reaction temperature was 52 ℃,the enzyme activity was 3?000U/g (sheep placenta) and the substrate concentration was 10%,the reaction time was 3 hours.The optimal heating condition was 100℃ for 15 minutes.