玉米醇溶蛋白/明胶/羟基磷灰石纳米纤维膜制备及其性质研究

本研究通过静电纺丝技术制备了玉米醇溶蛋白/明胶/纳米羟基磷灰石（nHAP）复合纳米纤维膜。通过旋转测试和振荡测试发现玉米醇溶蛋白/明胶/纳米羟基磷灰石溶液为剪切变稀非牛顿流体并且具有粘弹性。纳米羟基磷灰石的添加显著增加了玉米醇溶蛋白/明胶（3/1，w/w）纳米纤维的直径，但不同nHAP浓度的纳米纤维之间没有显著差异。高浓度nHAP的复合纳米纤维中观察到结节的形成，通过TEM观察发现nHAP在纤维内部和表面均有分布。红外光谱结果表明蛋白与nHAP之间通过氢键相互作用，当nHAP添加浓度为10%时引起了蛋白二级结构从β-折叠向转角结构的转变。玉米醇溶蛋白/明胶/纳米羟基磷灰石复合纳米纤维膜表现出疏水的表面，随着10%nHAP的加入疏水性有所增加，这是由于表面极性基团比例的下降。由于高浓度的nHAP在纤维中聚集导致其对纳米纤维膜的机械性能产生了不利影响。

Fabrication and characterization of zein/gelatin/nano-hydroxyapatite nanofibers

In this study, the zein/gelatin nanofibrous membranes with incorporation of nano-hydroxyapatite (nHA) were fabricated via electrospinning. The zein/gelatin/nHAP solutions as the spinning dispersions showed the viscoelastic property with a shear-thinning non-Newtown behavior. The incorporated nHAP increased the zein/gelatin nanofiber diameter, while no significant difference was observed between various nHAP content. Agglomeration and nodules at high nHAP contents were observed. It was found that nHAP was dispersed within the zein/gelatin nanofibers by the formed hydrogen bonding, resulting in a more compact structure of the proteins when adding 10% nHAP. The addition of nHAP increased the hydrophobicity of the nanofibrous membranes, due to the lower ratio of polar groups exposed outside. However, the incorporation of nHAP deteriorated and resulted in a more brittle and fragile property.