| 玉米须多糖中蛋白质脱除的Sevag与酶法联用工艺优化 |
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| 引用本文： | 周鸿立,杨晓虹.玉米须多糖中蛋白质脱除的Sevag与酶法联用工艺优化[J].食品科学,2011,32(8):129-132. |
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| 作者姓名： | 周鸿立 杨晓虹 |
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| 作者单位： | 1.吉林大学药学院
2.吉林化工学院化学与制药工程学院 |
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| 基金项目： | 吉林大学博士后基金项目(801070660432)；吉林省教育厅“十一五”科学技术研究项目(2010 第175 号) |
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| 摘 要： | 采用Sevag与酶法联用优化玉米须多糖脱蛋白的工艺。以脱蛋白率和多糖剩余率及综合评分为考察指标。结果表明，最佳工艺为酶底比2.5(mg/L)、温度50℃、振荡时间8min、脱蛋白次数2次。最终多糖纯度由21.88%提高为37.77%，脱蛋白率69.97%多糖，剩余率94.51%。此方法可用于玉米须多糖中蛋白质的脱除。
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| 关 键 词： | 玉米须 多糖 Sevag试剂 木瓜蛋白酶 脱蛋白 |
Removal of Proteins from Corn Silk Polysaccharide by a Combined Enzymatic-Sevag Method |
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| ZHOU Hong-li,YANG Xiao-hong.Removal of Proteins from Corn Silk Polysaccharide by a Combined Enzymatic-Sevag Method[J].Food Science,2011,32(8):129-132. |
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| Authors: | ZHOU Hong-li YANG Xiao-hong |
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| Affiliation: | 1. School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China；2. School of Chemical and Pharmaoeutical
Engineering, Jilin Institute of Chemical Technology, Jilin 132022 ,China |
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| Abstract: | Enzymatic hydrolysis followed by Sevag treatment was used to develop a combined method for removing proteins in crude corn silk polysaccharide. The enzyme used was papain. Its hydrolysis conditions were optimized by single factor and orthogonal array design methods based on deproteinization rate and the recovery of polysaccharides. The number of repeated Sevag treatment was also investigated. The optimal conditions for deprotenizing crude corn silk polysaccharide were as follows: enzyme-to-substrate ratio of 2.5 (mg/L), temperature of 50 ℃, oscillation time of 8 min and number of repeated Sevag treatment of 2. Under such conditions, the purity of crude corn silk polysaccharide was increased from 21.88% to 37.77%, and the deproteinizaiton rate and polysaccharide recovery were 69.97% and 94.51%, respectively. |
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| Keywords: | corn silk polysaccharide Sevag papain deproteinization |
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