| 香肠中红色2G的HPLC法及UPLC-MS/MS检测 |
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| 引用本文： | 凌云,储晓刚,孙利,张峰,王菡,陈琦,吴永宁.香肠中红色2G的HPLC法及UPLC-MS/MS检测[J].食品科学,2011,32(8):231-234. |
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| 作者姓名： | 凌云 储晓刚 孙利 张峰 王菡 陈琦 吴永宁 |
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| 作者单位： | 1.中国疾病预防控制中心营养与食品安全所
2.中国检验检疫科学研究院
3.沈阳农业大学植物保护学院
4.陕西科技大学生命科学与工程学院 |
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| 基金项目： | 北京市科技计划项目(D08050200310804) |
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| 摘 要： | 建立香肠中红色2G色素的高效液相色谱法(HPLC)测定和确证的超高压液相色谱-质谱/质谱法(UPLC-MS/MS)。以0.662mol/L氨水为提取液，样品均质提取两次，离心后用C18柱净化，采用高效液相色谱-二极管阵列检测器(HPLC-DAD)检测，流动相为乙酸-乙酸铵缓冲液-甲醇(55:45，V/V)，等度洗脱，流速为1mL/min，检测波长为530nm，阳性结果用UPLC-MS/MS确证。HPLC-DAD法在0.0212～1.06mg/L范围内，红色2G的峰面积与其相应浓度呈现良好相关性，r＞0.999，方法测定限为0.106mg/kg，在空白样品中添加已知浓度的红色2G色素，平均回收率在79.0%～86.5%之间，相对标准偏差(RSD)在3.19%～5.06%之间。UPLC-MS/MS法的测定限为0.005mg/kg，平均回收率在75.5%～80.2%之间，RSD在3.62%～9.97%之间。该方法可用于香肠中红色2G的检测及确证。
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| 关 键 词： | 红色2G 色素 高效液相色谱 超高压液相色谱串联质谱 确证 香肠 |
Determination of Red 2G in Sausage by High-performance Liquid Chromatography and Ultra Performance Liquid Chromatography-tandem Mass Spectrometry |
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| LING Yun,CHU Xiao-gang,SUN Li,ZHANG Feng,WANG Han,CHEN Qi,WU Yong-ning.Determination of Red 2G in Sausage by High-performance Liquid Chromatography and Ultra Performance Liquid Chromatography-tandem Mass Spectrometry[J].Food Science,2011,32(8):231-234. |
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| Authors: | LING Yun CHU Xiao-gang SUN Li ZHANG Feng WANG Han CHEN Qi WU Yong-ning |
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| Affiliation: | 1. Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China；
2. Chinese Academy of Inspection and Quarantine, Beijing 100025, China；
3. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China；
4. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi’an 712081, China |
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| Abstract: | A high-performance liquid chromatography method (HPLC) was developed for the determination of red 2G in sausage. Sausage samples were extracted twice with 0.662 mol/L NH3 ·H2O and the extract was cleaned up on a C18 column. Red 2G was analyzed by HPLC with diode array detector and confirmed by UPLC-MS/MS. The limit of quantification (LOQ) of HPLC was 0.106 mg/kg and the average recoveries ranged from 79.0% to 86.5% with RSD values between 3.19% and 5.06%. The LOQ of UPLC-MS/MS was 0.005 mg/kg and the average recoveries ranged from 75.5% to 80.2% with RSD values between 3.62% and 9.97%. The developed HPLC method and UPLC-MS method can be applied for the determination and confirmation of red 2G in sausage. |
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| Keywords: | red 2G color sausage high-performance liquid chromatography (HPLC) ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) confirmation |
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