毛细管电泳技术在乳制品营养与安全分析中的应用

乳制品的营养与安全一直广受世界范围内的关注,各种色谱及色谱-质谱联用技术在乳制品分析中得到广泛应用。毛细管电泳技术在乳制品的营养与安全分析中独具特色,成为不可或缺的分析技术。本文围绕毛细管电泳技术分析乳及乳制品中乳糖和半乳糖、胆碱、5’-单磷酸核苷酸、硝酸盐和亚硝酸盐、山梨酸和苯甲酸等小分子化合物的方法,结合本实验室利用毛细管电泳技术参加能力验证等方面的工作,从营养成分、限量物质、残留分析、禁用物质、复原乳识别和能力验证这6个方面,对近10年来毛细管电泳技术在乳制品营养与安全分析中有代表性的应用研究及其进展进行总结,并对其在乳制品中小分子化学物质分析中的发展趋势进行了展望。

Application of capillary electrophoresis in nutrition and safety analysis of dairy products

The nutrition and safety of dairy products have been widely concerned all over the world. Various chromatographic and hyphenated techniques coupled to mass spectrometry have been widely used in the analysis of dairy products. Capillary electrophoresis has its own characteristics in the nutrition and safety analysis of dairy products, and has become one of the indispensable analytical techniques. This paper focused on the analysis of small molecular compounds by capillary electrophresis, such as lactose and galactose, choline, 5-monophosphate nucleotides, nitrate and nitrite, sorbic acid and benzoic acid in milk and dairy products by capillary electrophoresis. The representative applications and progress of capillary electrophoresis in nutrition and safety analysis of dairy products in recent 10 years will be reviewed, combined with our laboratory''s work on proficiency test of milk drink by capillary electrophoresis, from the following 6 aspects: Nutritional composition analysis, limited substances, residue analysis, prohibited substances, reconstituted milk recognition and proficiency testing. The trends of capillary electrophoresis in the analysis of small molecular chemicals in dairy products was prospected.