桑黄胞内多糖免疫及抗氧化活性研究

通过腹腔注射环磷酰胺建立免疫抑制小鼠动物模型，考察桑黄粗多糖(SH)、纯化多糖组分(P-47000、P-8700)对增强免疫及抗氧化水平的变化情况，明确不同纯度多糖组分生物活性间的差异。结果表明：SH、P-47000、P-8700对环磷酰胺诱导的免疫功能低下小鼠具有不同程度的免疫促进作用，其顺序为：P-8700＞SH＞P-47000；P-47000的还原能力低于SH和P-8700，SH和P-8700还原能力相差不大；P-47000几乎对超氧阴离子自由基(O2－ ·)没有清除效果，P-8700和SH的清除率比较低，且P-8700略大于SH；P-8700对羟自由基( ·OH)具有较好的清除效果，清除率达到53.421%，且P-8700的清除率高于SH和P-47000，P-47000清除率最低；不同纯度多糖对DPPH自由基的清除效果均较高，SH达到93.221%；P-8700清除效果最低，为66.435%。

Immune Function and Antioxidant Activity of Intracellular Polysaccharides from Phellinus baumii

An immune-deficient mouse model was established by intraperitoneal injection of cyclophosphamide (CTX) to explore the immune enhancement and antioxidant capability of crude polysaccharides (SH) from Phellinus banmii and purified polysaccharide fractions (P-47000 and P-8700). Meanwhile, the bioactivity differences among SH, P-47000 and P-8700 were investigated. The results showed that SH, P-47000, and P-8700 could enhance immune function of CTX-induced immune-deficient mice to some extent. The order of immune enhancement function from strong to weak was P-8700, SH and P-47000. P-47000 exhibited a lower reducing power than SH and P-8700. However, no significant difference in reducing power between SH and P-8700 was observed. In addition, P-47000 revealed lower ability to scavenge superoxide anion free radicals than SH and P-8700. Moreover, P-8700 revealed stronger hydroxyl free radical scavenging capacity with a scavenging rate of 53.421%, which was obviously higher than that of SH and P-47000. All the polysaccharides had high scavenging capability against DPPH free radicals. SH and P-8700 revealed the highest and lowest scavenging capability on DPPH free radicals with a scavenging rate of respectively 93.221% and 66.435%.