高效液相色谱法测定芦荟甙的4个标准方法比较

目的比较4个标准方法中高效液相色谱法测定芦荟及其制品中芦荟甙含量的优劣。方法分别采用4个标准的前处理方法处理芦荟及其制品,并对样品做低、中、高3个浓度水平的加标实验,采用4个色谱方法测定芦荟甙的含量。通过计算相对标准偏差和回收率等方面来评价4个方法的优劣。结果芦荟甙的出峰时间为8.807 min。线性方程为Y=13700X+16000,相关系数r为0.9994。4种方法的相对标准偏差为0.21%、0.37%、0.35%和0.18%,加标回收率为97.3%、98.3%、98.8%和98.5%。结论 4个方法的测定结果均满足对芦荟及其制品测定芦荟甙前处理要求,其中QB/T2489-2007《食品原料用芦荟制品》的前处理步骤最方便快捷,使用的有机试剂量最少,回收率最高。适用于芦荟及其制品中芦荟甙含量的测定。

Comparison of 4 standard methods for determination of aloin by high performance liquid chromatography

Abstract: Objective Determination of aloin in aloe and its products by high performance liquid chromatography, and the methods of four kinds of aloe and its products were compared to provide reference for selecting the best method. Methods Four standard pretreatment methods were used to treat aloe and its products respectively, and standard addition experiments of low, medium and high concentrations were carried out on samples to determine the content of aloin. The relative standard deviation and recovery rate were calculated to evaluate the advantages and disadvantages of the four methods. Result The experimental results show that the mobile phase methanol :1% acetic acid water=50: 50(V/V); The flow rate was 1.0 mL/min and the peak time of aloin was 8.901min. The linear equation was Y=13700X+16000, and the correlation coefficient r is 0.9994. The relative standard deviations of the four methods were 0.21%, 0.37%, 0.35% and 0.18%, and the recoveries were 97.3%, 98.3%, 98.8% and 98.5%. Conclusion The determination results of the four methods all meet the pre-treatment requirements for the determination of aloin in aloe and its products. Among them, QB/T 2489-2007 aloe products for food raw materials have the most convenient and fast pre-treatment steps, use the least amount of organic test and have the highest recovery rate. It is suitable for the quantitative detection of aloin in aloe and its products.