毛细管电泳技术在乳品蛋白分析中的应用

乳品在全球范围内生产和消费,受到广泛且持续的关注,蛋白质是乳品评价最重要的指标之一。毛细管电泳技术(capillary electrophoresis, CE)具有样品用量少、分离模式多、分析效率高的优势,在乳品蛋白质分析方面具有巨大的应用潜力。本文简要介绍了CE技术检测乳品中蛋白质的特点和难点,总结了毛细管凝胶电泳法、涂层毛细管电泳法、胶束电动毛细管色谱法、免疫亲和毛细管电泳法、毛细管区带电泳法等模式在乳品蛋白质分析中的优劣势,列举了CE技术在乳品品质鉴定、过敏原分析、真伪鉴别、蛋白质糖基化分析、乳品处理和蛋白水解过程等相关生产和质控环节的应用情况,展望了CE技术在乳品分析应用中的发展趋势。

Application of capillary electrophoresis in protein analysis of dairy products

The production and consumption of dairy products in the world has received extensive and sustained attention. Protein is one of the most important indicators for dairy product evaluation. Capillary electrophoresis (CE) has the advantages of less sample consumption, more separation modes and higher analysis efficiency, so it has great potential in the analysis of dairy protein. This paper briefly introduced the characteristics and difficulties of CE technology in the detection of protein in dairy products, summarized the advantages and disadvantages of capillary gel electrophoresis, coated capillary electrophoresis, micellar electrokinetic capillary chromatography, immunoaffinity capillary electrophoresis, capillary zone electrophoresis and other models in dairy protein analysis. It listed the application of CE technology in milk quality identification, allergen analysis, authenticity identification, protein glycosylation analysis, milk treatment and protein hydrolysis process and other related production and quality control processes. It also prospected the development trend of CE technology in dairy analysis.