高效液相色谱法测定南、北五味子中4种木脂素含量

目的：采用高效液相色谱法同时测定17种不同产地的五味子中五味子醇甲、五味子醇乙、五味子甲素和五味子乙素含量。方法：采用Diamonsil C18(250mm×4.6mm，5μm)柱，甲醇-水(73:27，V/V)作流动相，流速1.0mL/min，检测波长254nm。结果：五味子醇甲、五味子醇乙、五味子甲素和五味子乙素含量测定方法的线性关系良好，线性范围为0.01～0.08mg/mL，相关系数R2＞0.9990；RSD分别为0.86%、0.37%、0.19%和0.15%。结论：该方法精密度、重现性和分离效果好，分析快速准确，适合于该药材中五味子醇甲、五味子醇乙、五味子甲素和五味子乙素含量的测定；不同产地的4种木脂素成分均存在一定的差别，北五味子中五味子醇甲、五味子醇乙和五味子乙素的含量稍高，而五味子甲素含量却稍低，南五味子恰与之相反。

Determination of Lignins in Schisandra sphenanthera and Schisandra chinensis by High Performance Liquid Chromatography

Objective: To establish a HPLC method for the determination of schisandrin, schisandrol B, deoxyschizandrin and schisandrin B in Schisandra sphenanthera Rehd.et Wils. and Schisandra chinensis (Turcz.) Baill. from 17 growth areas. Methods: The chromatographic separation was carried out on Diamonsil C18 (250 mm× 4.6 mm, 5 μm) column using methanol-water (73:27, V/V) as the mobile phase at a flow rate of 1 mL/min. The detection wavelength was 250 nm. Results: A good linear relationship was achieved with a correlation coefficient in the range of 0.01 to 0.08 mg/mL (R2 = 0.9990) and a RSD of 0.86%, 0.37%, 0.19% and 0.15%, respectively. Conclusion: The developed HPLC method reveals a good separation efficiency, reproducibility and accuracy. This method can be used for determining schisandrin, schisandrol B, deoxyschizandrin and schisandrin B in herbs. Higher amounts of schisandrin, schisandrol B and schisandrin B are present in Schisandra chinensis than in Schisandra sphenanthera.