芦苇叶类黄酮高效液相色谱分析

目的：分析芦苇叶类黄酮的组分。方法：制备芦苇叶类黄酮提取浓缩过程中形成的析出物和浓缩液再经大孔树脂纯化后产生的初纯物，优化两种样品中类黄酮苷转化为苷元的水解条件，采用高效液相色谱(HPLC)法测定两种样品和其水解样品类黄酮的组分。结果：析出物和初纯物的总黄酮含量分别为57.1%和18.5%，酸浓度1.4mol/L、温度80～90℃条件下水解4h可获得相对最好的水解效果；HPLC测定表明，析出物含32种组分，其中7种得到确定，初纯物含22种组分，其中5种得到确定；水解析出物含28种组分，其中7种得到确定，水解初纯物含25种组分，其中7种得到确定。结论：芦苇叶含有芦丁、野黄芩苷、橙皮苷、木犀草素、槲皮素、芹菜素、山奈酚、异鼠李素(或橙皮素)、异甘草素和黄芩素，其中芹菜素含量最高，还有许多未确定的类黄酮。

HPLC Analysis of Flavonoids from Reed Leaves

Objective: To analyze the compositions of flavonoids from reed leaves. Methods: The crude extract was prepared during the condensation of reed leaf extract. The primarily purified flavonoids were prepared by macro-porous resin. The hydrolysis conditions of flavonoids were optimized. Two samples were hydrolyzed to obtain two hydrolysis products. The compositions of flavonoids were determined by HPLC. Results: The contents of total flavonoids in crude extract and primary purified flavonoids samples were 57.1% and 18.5%, respectively. The optimal hydrolysis conditions for reed leaf flavonoids were hydrolysis temperature of 80－90 ℃, HCl concentration of 1.4 mol/L and hydrolysis time of 4 h. Totally 33 components were separated by HPLC from the crude extract and 8 components were identified based on their retention time. A total of 22 components were separated by HPLC from the primarily purified sample and 5 components were identified. A total of 28 components were separated from the hydrolysates and 7 components were identified. Similarly, 25 components were separated from the hydrolyzed primarily purified samples and 7 components were identified. Conclusion: Reed leaves contained rutin, scutellarin, hesperidin, luteolin, quercetin, apigenin, kaempferol, isorhamnetin or hesperetin, isoliquiritigenin, baicalein, and other unknown flavonoids. The content of apigenin among these components was the highest.