| 响应面法优化重组大肠杆菌PUCRF发酵培养基的研究 |
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| 引用本文： | 王 华,于寒松,朴春红,刘俊梅,胡耀辉. 响应面法优化重组大肠杆菌PUCRF发酵培养基的研究[J]. 食品科学, 2011, 32(9): 225-230. DOI: 10.7506/spkx1002-6630-201109051 |
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| 作者姓名： | 王 华 于寒松 朴春红 刘俊梅 胡耀辉 |
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| 作者单位： | 1.吉林农业大学食品科学与工程学院2.内蒙古民族大学生命科学学院 |
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| 基金项目： | 吉林省科技发展计划项目(20086030) |
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| 摘 要： | 为进一步提高重组大肠杆菌( PUCRF)产β-环糊精葡萄糖基转移酶(β-CGTase)的酶活力，以构建的一株能在胞外分泌表达β-CGTase工程菌E.coli PUCRF 为出发菌株，利用响应面法对其发酵工艺条件进行优化。根据Box-Benhnken中心组合设计原理，在单因素试验基础上采用四因素三水平的响应面分析法确定重组大肠杆菌PUCRF最佳培养基组成(g/100mL)为：玉米淀粉 0.962、玉米浆0.097、MgSO4 ·7H2O 0.153、K2HPO4 ·3H2O 1.235。经过优化，β-CGTase产量可达3610U/mL，是初始条件下β-CGTase酶活力(2690U/mL)的1.34倍。
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| 关 键 词： | 响应面 β-环糊精葡萄糖基转移酶 发酵 培养基 |
Optimization of Culture Medium for Recombinant Escherichia coli PUCRF Using Response Surface Analysis |
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| WANG Hua,YU Han-song,PIAO Chun-hong,LIU Jun-mei,HU Yao-hui. Optimization of Culture Medium for Recombinant Escherichia coli PUCRF Using Response Surface Analysis[J]. Food Science, 2011, 32(9): 225-230. DOI: 10.7506/spkx1002-6630-201109051 |
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| Authors: | WANG Hua YU Han-song PIAO Chun-hong LIU Jun-mei HU Yao-hui |
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| Affiliation: | 1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China；2. College of Life Science, Inner Mongolia University for the Nationalities, Tongliao 028000, China |
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| Abstract: | An engineered strain E. coli PUCRF producing β-cyclodextrin glycosyltransferase (β-CGTase) has constructed in our lab. In this study, response surface analysis was used to optimize the culture medium composition for the production of β-CGTase by the strain. On the basis of one-factor-at-a-time experiments, a 4-factor, 3-level central composite design was performed. The optimum culture medium composition (g/100 mL) was determined as follows: corn starch 0.962, corn syrup 0.097, MgSO4 ·7H2O 0.153 and K2HPO4 ·3H2O 1.235. The correspondingβ-CGTase yield was 3610 U/mL, 1.34 times higher than before the optimization (2690 U/mL). |
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| Keywords: | response surface analysis β-cyclodextrain glycolstransferase fermentation medium |
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