| 6个不同品系地木耳抗氧化酶活性比较研究 |
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| 引用本文： | 冯 佳,吴 琴,李 砧,谢树莲.6个不同品系地木耳抗氧化酶活性比较研究[J].食品科学,2011,32(11):107-109. |
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| 作者姓名： | 冯 佳 吴 琴 李 砧 谢树莲 |
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| 作者单位： | 1. 山西大学生命科学学院
2.太原师范学院生物系 |
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| 基金项目： | 山西省农业科技攻关项目(2006031036)；山西省工业科技攻关项目(20100321107) |
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| 摘 要： | 目的：通过对6个不同品系地木耳抗氧化酶活性的比较和分析，筛选抗逆性较高的品系。方法：采用紫外分光光度法测定过氧化氢酶(CAT)，采用分光光度法测定抗坏血酸过氧化物酶(APX)，采用DTNB染色法测定谷胱甘肽过氧化物酶(GSH-PX)。结果：山西宁武荷叶坪品系CAT活性最大，且与其他品系之间存在显著差异(P＜0.05)；山西宁武秋千沟品系GSH-PX、APX活性均明显大于其他品系(P＜0.05)。结论：从抗氧化酶体系来看，秋千沟品系的抗逆性优于其他品系抗逆性。
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| 关 键 词： | 地木耳 过氧化氢酶（CAT） 抗坏血酸过氧化酶（APX） 谷胱甘肽过氧化物酶（GPX） |
Comparison on Antioxidant Enzyme Activities in Six Strains of Nostoc commune |
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| FENG Jia,WU Qin,LI Zhen,XIE Shu-lian.Comparison on Antioxidant Enzyme Activities in Six Strains of Nostoc commune[J].Food Science,2011,32(11):107-109. |
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| Authors: | FENG Jia WU Qin LI Zhen XIE Shu-lian |
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| Affiliation: | 1. College of Life Science, Shanxi University, Taiyuan 030006, China；
2. Department of Biology, Taiyuan Normal University, Taiyuan 030031, China |
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| Abstract: | Objective: To compare antioxidant enzyme activities in 6 strains of Nostoc commune and screen the strain with higher stress resistance. Methods: Catalase (CAT) and ascorbate peroxidase (APX) were determined by spectrophotometry. Glutathione peroxidase (GSH-PX) was determined by DTNB staining method. Results: the highest activity of CAT was determined in Heyeping strain, which revealed a significant difference from the other five. In addition, Qiuqiangou stain revealed significantly higher activities of GSH-PX and APX than the other strains. Conclusion: Qiuqiangou strain has higher stress resistance than the other strains investigated on the basis of antioxidant analysis. |
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| Keywords: | Nostoc commune catalase (CAT) ascorbate peroxidase (APX) glutathione peroxidase (GSH-PX) |
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