高通量全自动仪器法测定植物源性食品中甲基托布津残留量

建立高效的应用高通量全自动均质仪、全自动净化在线浓缩仪及液相色谱-串联质谱仪测定植物源性食品中甲基托布津残留量的检测方法。试样经高通量全自动均质仪，用乙腈均质提取，经全自动净化在线浓缩仪(凝胶色谱柱、固相萃取柱)净化、在线浓缩后，采用液相色谱-串联质谱仪定量定性分析，外标法定量，液相流动相为甲醇-水，采用电喷雾离子源，定性离子对为m/z 343.0＞151.1、m/z 343.0＞192.1，定量离子对为m/z 343.0＞151，添加0.01～10.0mg/kg的甲基托布津标准品时的回收率为88.4%～97.9%，相对标准偏差(RSD)在1.3%～2.9%之间，最低检出限为0.01mg/kg。该方法特异性强、敏感性高、高通量、全自动、快速高效，可应用于植物源性食品的甲基托布津残留量检测。

High-throughput Automated Instrumental Analysis of Thiophanate-methyl Residue in Plant-derived Foods

An efficient detection method for thiophanate methyl residue in plant-derived foods was established by combing a high-throughput automated homogenizer, an automatic online purification and concentration system and a liquid chromatography-tandem mass spectrometry. Samples were homogenized with acetonitrile on a high-throughput automated homogenizer, purified using an automatic online purification (gel chromatographic and solid phase extraction column) system, online concentrated and detected using a liquid chromatography-tandem mass spectrometer. The quantification of thiophanate methyl was carried out using the external standard method. The detection conditions were: acetonitrile-water (gradient) as mobile phase, ESI as ionization method, qualitative ion pairs of m/z 343.0＞151.1 and 343.0＞192.1, quantitative ion pairs of m/z 343.0＞151.1. With the addition of 0.01 to 10.0 mg/kg of thiophanate standard to samples, the recovery rate was between 88.4% and 97.9% and the relative standard deviations (RSDs) varied from 1.3% to 2.9%. The detection limit of the established method was 0.01 mg/kg.