| 高压二氧化碳和超高压对多酚氧化酶和果胶甲酯酶结构的影响 |
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| 引用本文： | 刘 野,张 超,赵晓燕,胡小松.高压二氧化碳和超高压对多酚氧化酶和果胶甲酯酶结构的影响[J].食品科学,2011,32(11):83-87. |
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| 作者姓名： | 刘 野 张 超 赵晓燕 胡小松 |
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| 作者单位： | 1.北京工商大学化学与环境工程学院
2.北京市农林科学院蔬菜研究中心
3.中国农业大学食品科学与营养工程学院 |
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| 基金项目： | 国家“863”计划项目(2007AA100405)；北京市重大科技计划项目(101105046610000) |
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| 摘 要： | 为考察高压二氧化碳(HPCD)和超高压技术钝化多酚氧化酶(PPO)和果胶甲酯酶(PE)活性的机理，采用SDS-PAGE电泳、红外光谱和荧光光谱对处理前后酶蛋白的结构进行比较研究。结果表明：经过不同压强HPCD处理和超高压处理60min后，PPO和PE的活性均随着处理压强的增大而逐渐降低。SDS-PAGE电泳分析显示处理后酶蛋白的肽链不发生断裂，但红外光谱和荧光光谱分析显示酶蛋白的二级和三级结构均发生变化。因此，HPCD和超高压技术通过改变PPO和PE酶蛋白的结构达到钝化酶活性的目的。
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| 关 键 词： | 多酚氧化酶 高压二氧化碳 果胶甲酯酶 结构 超高压 |
Effect of High Pressure Carbon Dioxide and Ultra-high Pressure on Structure of Polyphenol Oxidase and Pectin Methylesterase |
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| LIU Ye,ZHANG Chao,ZHAO Xiao-yan,HU Xiao-song.Effect of High Pressure Carbon Dioxide and Ultra-high Pressure on Structure of Polyphenol Oxidase and Pectin Methylesterase[J].Food Science,2011,32(11):83-87. |
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| Authors: | LIU Ye ZHANG Chao ZHAO Xiao-yan HU Xiao-song |
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| Affiliation: | 1. College of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100048, China；
2. Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China；
3. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | In order to investigate the mechanism of high pressure carbon dioxide (HPCD) and ultra-high pressure inactivating polyphenol oxidase (PPO) and pectin methylesterase (PE), SDS-PAGE, infrared spectroscopy, and fluorescence spectroscopy were applied to study the structural change of PPO and PE after the treatments. After HPCD and ultra-high pressure treatments for 60 min, the activity of PPO and PE decreased as the pressure ascent in the investigated range. SDS-PAGE analysis showed that no peptide chains were cut off, but according to the infrared and fluorescence spectral profiles the protein secondary and tertiary structures of the two enzymes were changed after the treatments. Hence, HPCD and ultra-high pressure inactivate PPO and PE through changing their advanced structures. |
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| Keywords: | high pressure carbon dioxide (HPCD) ultra-high pressure polyphenol oxidase (PPO) pectin methylesterase (PE) structure |
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