铜绿假单胞菌M19降解黄曲霉毒素B1的产物研究（网络首发、推荐阅读）

以实验室筛选保藏的黄曲霉毒素B1（Aflatoxin B1，AFB1）降解菌M19产生的降解酶PADE为对象，对其AFB1降解液进行薄层色谱及荧光光谱分析（LC-MS），分析降解产物可能的结构变化，并利用液相质谱检测AFB1降解产物。薄层色谱检测结果：有机相和水相降解液均未检测到新的荧光吸收物质，表明降解产物没有荧光吸收；荧光光谱检测结果：AFB1降解产物的荧光明显减弱；液相质谱检测结果：发现质荷比为227.18的物质P。结果推断：AFB1降解过程中内酯键断裂，产生一个分子量为226的降解产物P，利用Xcalibur软件分析其分子式为C14H10O3，并根据AFB1降解后的LC-MS图谱分析以及AFB1降解产物的相关文献对降解途径进行假设。

Analysis of the Degradation Products of Aflatoxin B1 by Pseudomonas aeruginosa M19(Online First, Recommended Article)

A degrading enzyme PADE produced during the degradation of aflatoxin B1 (AFB1) by the laboratory screening bacteria M19 was selected as the subject in this study. Thin layer chromatography and fluorescence spectrum analysis were performed to analyze possible structural changes of products in the AFB1 degradation solution. Mass spectrometric were further used to detect the AFB1 degradation products. The results of thin layer chromatography showed that no new fluorescent absorbent substance was detected in the organic phase degradation solution, and no absorbent substance was detected in the aqueous phase degradation solution, indicating that the degradation product did not have fluorescence absorption. The fluorescence spectrum detection results showed that the fluorescence of the AFB1 degradation product was decreased obviously. The mass spectrometry results showed that a substance P with a mass-to-charge ratio of 227.18 was found. Based on all the results, it can be concluded that the lactone bond was broken during the degradation of AFB1, and a degradation product P with a molecular weight of 226 was generated. The molecular formula was C14H10O3 as analyzed by Xcalibur software. The degradation pathway was hypothesized.