| 肉鸡中魏斯菌属细菌的分离与鉴定 |
| |
| 引用本文： | 纪淑娟,李 丽,王金玲.肉鸡中魏斯菌属细菌的分离与鉴定[J].食品科学,2011,32(9):164-166. |
| |
| 作者姓名： | 纪淑娟 李 丽 王金玲 |
| |
| 作者单位： | 1.沈阳农业大学食品学院
2.沈阳出入境检验检疫局 |
| |
| 基金项目： | 沈阳出入境检验检疫局大型科学仪器设备共享服务项目(090161) |
| |
| 摘 要： | 应用16S DNA基因测序法对肉鸡中分离出的可疑魏斯菌属细菌菌株进行鉴定。从沈阳某养鸡场抽取病死鸡在无菌条件下采集样品10份，微需氧条件下进行细菌分离纯化，比对魏斯菌属的基本形态和革兰氏染色特点，得到与魏斯菌属类似菌株14株。采用16S rDNA基因测序方法对分离出的细菌进行基因序列分析。在NCBI网站用BLAST软件在GenBank上进行同源性比较，比对结果表明编号为X1001、X1002、X1006、X1008、X1009的株菌同源性达90%。生化实验结果表明，X1006号菌株为魏斯菌属中的融合魏斯菌属菌株。
|
| 关 键 词： | 肉鸡 魏斯菌属细菌 分离 鉴定 基因测序 |
Isolation and Identification of Weissella Strains in Chicken |
| |
| JI Shu-juan,LI Li,WANG Jin-ling.Isolation and Identification of Weissella Strains in Chicken[J].Food Science,2011,32(9):164-166. |
| |
| Authors: | JI Shu-juan LI Li WANG Jin-ling |
| |
| Affiliation: | 1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China；
2. Shenyang Entry-Exit Inspection and Quarantine Bureau, Shenyang 110016, China |
| |
| Abstract: | The suspicious Weissella strains isolated from chicken were identified by 16S rDNA gene sequencing. Under sterile conditions, 10 dead broiler samples were collected from a certain chicken farm in Shenyang. The bacteria in each sample were isolated and purified under micro-aerobic conditions. Totally, 14 bacterial strains were obtained and identified as Weissella. PCR based 16S rDNA gene sequencing showed that X1001, X1002, X1006, X1008 and X1009 strains had high homology (above 90%). The biochemical experimental results revealed that X1006 strain had the characteristics of Weissella confusa. |
| |
| Keywords: | chicken Weissella isolation identification gene sequencing |
|
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
