固相萃取-高效液相色谱检测葡萄酒中罗丹明B

建立固相萃取富集净化，高效液相色谱紫外检测葡萄酒中禁用色素罗丹明B的方法。葡萄酒样品中的罗丹明B经C18固相萃取，40%乙醇溶液清洗净化，80%的甲醇溶液洗脱后，用高效液相色谱紫外检测器进行测定。结果表明：罗丹明B在1.0～10.0μg/mL范围呈现良好的线性关系，相关系数0.9924；在1.5～3.5μg/mL添加水平时，平均回收率为72.06%，相对标准偏差为2.87%(n=5)；定量测出限为0.25μg/mL。表明该方法灵敏度高，重复性良好，操作简便快速，适用于葡萄酒中罗丹明B的检测。

Determination of Rhodamine B in Red Wine by Solid Phase Extraction-High Performance Liquid Chromatography

A solid phase extraction followed by high performance liquid chromatographic method was developed for the analysis of the prohibited colorant rhodamine B in red wine. The separation of rhodamine B from wines was achieved on a Supelclean- C18 SPE column washed with 40% ethanol aqueous solution (V/V) and eluted with 80% methanol aqueous solution (V/V) prior to analysis on a high performance liquid chromatography equipped with a UV detector. The calibration curve of the method was linear over the range of 1.0 to 10.0μg/mL with a correlation coefficient of 0.9924. The recoveries for rhodamine B standard spiked in the range of 1.5 to 3.5μg/mL were between 69.67% and 75.00% with a relative standard deviation (RSD) of 2.87% (n = 5). The detection limit was 0.25μg/mL. Owing to advantages of good linearity, low limit of detection, high precision and rapidity, this method is suitable for the determination of rhodamine B in red wine.