| 裂殖弧菌DHA油脂纯化后的氧化稳定性 |
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| 引用本文： | 龚东平,陈园力,张红漫,胡耀池,黄 和. 裂殖弧菌DHA油脂纯化后的氧化稳定性[J]. 食品科学, 2011, 32(11): 79-82. DOI: 10.7506/spkx1002-6630-201111018 |
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| 作者姓名： | 龚东平 陈园力 张红漫 胡耀池 黄 和 |
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| 作者单位： | 1. 南京工业大学生物与制药工程学院，材料化学工程国家重点实验室2.南京工业大学理学院 |
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| 基金项目： | 国家自然科学基金重点项目(20936002)；国家“973”计划项目(2007CB707805；2009CB724700)；江苏省六大人才高峰项目(2008)；江苏省高校科研成果产业化推进项目(2009) |
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| 摘 要： | 对裂殖弧菌DHA油脂进行纯化，研究其氧化稳定性的变化。DHA油脂经多层填料层析柱纯化后，分析纯化前后DHA油脂的脂肪酸组成及微组分的含量变化，并对其甲醇萃取物进行DPPH自由基清除能力的评价。纯化前后的DHA油脂采用光照和Schaal烘箱法进行加速氧化，以过氧化值(POV，一级氧化指标)和硫代巴比妥酸反应物(TBARS，二级氧化指标)表征油脂氧化情况。结果表明：DHA油脂经纯化去除微组分后，其甲醇萃取物清除 DPPH自由基的能力以及对光、对热的稳定性均显著降低。
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| 关 键 词： | DHA油脂 纯化 微组分 DPPH 氧化稳定性 |
Oxidative Stability of Purified DHA Oil from Schizochytrium sp. |
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| GONG Dong-ping,CHEN Yuan-li,ZHANG Hong-man,HU Yao-chi,HUANG He. Oxidative Stability of Purified DHA Oil from Schizochytrium sp.[J]. Food Science, 2011, 32(11): 79-82. DOI: 10.7506/spkx1002-6630-201111018 |
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| Authors: | GONG Dong-ping CHEN Yuan-li ZHANG Hong-man HU Yao-chi HUANG He |
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| Affiliation: | 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology, Nanjing 210009, China；2. College of Science, Nanjing University of Technology,Nanjing 210009, China |
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| Abstract: | The oxidative stability of purified DHA oil from Schizochytrium sp. was studied. The change of fatty acid composition and minor components in DHA oil was determined by the multilayer chromatography. Methanol extract of DHA oil was also investigated for its 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenge activity. The oxidative stability of purified and non-purified DHA oil under light and Schaal oven conditions was monitored by measuring peroxide value (PV) and thiobarbituric acid reactive substances (TBARS). Results showed that DPPH radical scavenging ability of methanol extract and the oxidative stability of DHA oil decreased significantly after removal of minor compounds by methanol extraction. |
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| Keywords: | DHA oil purification minor compounds DPPH radical oxidative stability |
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