超高效液相色谱-串联质谱法分析测定食用植物油中痕量δ9-四氢大麻酚

以电喷雾离子源(ESI)为电离源，在负离子采集模式下建立食用植物油中δ9四氢大麻酚(δ9-THC)超高效液相色谱-质谱/质谱(UPLC-MS/MS)检测方法。实验以甲醇为提取剂，经中性氧化铝固相萃取柱净化、Waters ACQUITYTM UPLC BEH-C18(2.1mm×100mm，1.7μm)色谱柱分离后以UPLC-MS/MS多反应监测模式进行分析。方法检出限和定量限分别为0.15μg/kg和0.45μg/kg。实验以δ9-氘代四氢大麻酚(δ9-THC-D3)为内标物，采用内标法定量。3个添加水平，平均回收率为68.0%～110.0%，相对标准偏差为8.8%～18.1%。本方法能够满足食用植物油中δ9-四氢大麻酚痕量检测的要求。

Determination of THC in Edible Vegetable Oil by Ultra-high Performance Liquid Chrmatography-Electrospray Tandem Mass Spectrometry

A determination method for δ9-tetrahydrocannabinol (THC) in edible vegetable oil was developed on the basis of ultra-high performance liquid chrmatography-electrospray tandem mass spectrometry (UPLC-MS/MS) under negative ion mode using electrospray ionization (ESI) source. THC was extracted with methanol and then purified by LC-alumina-N solid phase extraction column. Qualitative and quantitative analysis were carried out for the analyte under a MRM mode after THC was separated on Waters ACQUITY UPLCTM BEH C18 (1.7 μm, 2.1 mm×100 mm) column. The limit of detection (LOD) of the developed method was 0.15 μg/kg and the limit of quantification (LOQ) was 0.45 μg/kg for THC. Quantitative analysis was corrected by internal standard method using delta 9-THC-D3 as internal standard. Average recovery rate of THC varied from 68.0%－110.0% with relative standard deviation ranging from 8.8%－18.1% at three spiked levels. Therefore, the developed method meets the requirements for the determination of THC in edible vegetable oil.