| 一株产木糖醇菌株的生理生化及分子生物学鉴定 |
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| 引用本文： | 曲佳乐,闵伟红,王巧玲,方 丽.一株产木糖醇菌株的生理生化及分子生物学鉴定[J].食品科学,2011,32(11):171-175. |
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| 作者姓名： | 曲佳乐 闵伟红 王巧玲 方 丽 |
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| 作者单位： | 吉林农业大学食品科学与工程学院 |
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| 基金项目： | 长春市科技局应用技术研究计划项目(09YJ32) |
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| 摘 要： | 对一株未经报道的转化木糖为木糖醇的菌株CTD249，采用生理生化及分子生物学方法进行鉴定，同时构建系统发育树进行分析，确定菌株CTD249的种属。结果表明：CTD249与季也蒙毕赤酵母(Pichia guilliermondii)处于同一进化分支中，相似度达99%以上，因此将该菌株鉴定为季也蒙毕赤酵母(GenBank接受号：HM236292)。该菌株在初始木糖质量浓度20g/L的条件下，发酵48h后木糖醇产量为12.0g/L。
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| 关 键 词： | 木糖醇 季也蒙毕赤酵母 系统发育分析 鉴定 |
Physiological,Biochemical and Molecular Biological Identification of a Xylitol-producing Strain |
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| QU Jia-le,MIN Wei-hong,WANG Qiao-ling,FANG Li.Physiological,Biochemical and Molecular Biological Identification of a Xylitol-producing Strain[J].Food Science,2011,32(11):171-175. |
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| Authors: | QU Jia-le MIN Wei-hong WANG Qiao-ling FANG Li |
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| Affiliation: | College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | In this paper, an unreported strain (CTD249) with the capability of transforming xylose to xylitol was identified by physiological, biochemical and molecular biological methods. Meanwhile, a phylogenetic tree was constructed to analyze the genus which the strain CTD249 belongs to. The results indicated that the strain CTD249 and Pichia guilliermondii were in the same branch with more than 99% sequence similarity. This strain was identified as Pichia guilliermondii. With the initial xylose concentration of 20 g/L, the output of xylitol was up to 12.0 g/L after 48 h cultivation. |
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| Keywords: | xylitol Pichia guilliermondii phylogenetic analysis identification |
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