Effects of a (n?3) polyunsaturated fatty acid-deficient diet on profiles of serum vitellogenin and lipoprotein in vitellogenic trout (Salmo gairdneri) |
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Authors: | L Frémont C Léger B Petridou M T Gozzelino |
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Affiliation: | (1) Station de Recherches de Nutrition, C.N.R.Z.-I.N.R.A., 78350 Jouy-en-Josas, France |
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Abstract: | During the 6 months of vitellogenesis, 3-year-old female trout (Salmo gairdneri) were fed either an enriched (E) or an (n−3) polyunsaturated fatty acid (PUFA)-deficient (D) diet; serum vitellogenin (VG)
and lipoproteins (d<1.21 g/ml) were analyzed at the third month of vitellogenesis (September) and at ovulation (December).
The serum content of high density lipoproteins (HDL), the major protein class, maintained a mean value of 1500 mg/dl at both
stages and with both diets. On the contrary, very low density lipoproteins (VLDL) were 90% higher during vitellogenesis than
at spawning time, whereas excess vitellogenin circulated at this period (6580 mg/dl serum with diet E). The diet deficient
in (n−3) lowered serum vitellogenin content by 16% in September and by 26% in December. The degree of (n−3) PUFA incorporation
moderately decreased in low density lipoproteins (LDL) and in HDL with the (n−3)-deficient diet. The effect was more pronounced
for 20∶5. On the other hand, essential 22∶6 was incorporated into vitellogenin at the same rate in September as in December
with diet E (23% and 25%, respectively), whereas after a 3-month deficiency, the percentage fell to 12%; this percentage rose
again to 19% at spawning time. These findings show that, although stored (n−3) PUFA were not exhausted after a 6-month dietary
deficiency, the incorporation of essential fatty acids (EFA) into vitellogenin during the early stages of oogenesis was low,
suggesting changes in egg composition that may influence hatching. |
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