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Characterization of the {beta}-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies |
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| Authors: | Alkema Wynand BL; Hensgens Charles MH; Kroezinga Els H; de Vries Erik; Floris Rene; van der Laan Jan-Metske; Dijkstra Bauke W; Janssen Dick B |
| Affiliation: | 1 Department of Biochemistry and 3 Laboratory of Biophysical Chemistry, BIOSON Research Institute, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen and 4 DSM – Gist, PO Box 1, 2600 MA Delft, The Netherlands |
| Abstract: | The binding of penicillin to penicillin acylase was studiedby X-ray crystallography. The structure of the enzyme–substratecomplex was determined after soaking crystals of an inactiveßN241A penicillin acylase mutant with penicillin G.Binding of the substrate induces a conformational change, inwhich the side chains of |
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