| 木醋杆菌的培养基优化研究 |
| |
| 引用本文： | 杜晶晶,曹献英,林强,陈胜杰,胡凌俊. 木醋杆菌的培养基优化研究[J]. 纤维素科学与技术, 2011, 19(1): 35-40 |
| |
| 作者姓名： | 杜晶晶 曹献英 林强 陈胜杰 胡凌俊 |
| |
| 作者单位： | 海南省优势资源化工材料应用技术教育部重点实验室;海南大学材料与化工学院,海南,海口,570228 |
| |
| 基金项目： | 国家自然科学基金，海南省自然科学基金，海南大学科研项目 |
| |
| 摘 要： | 采用六因素三水平正交实验对木醋杆菌Acetobacter xylinum HN001的培养基进行了优化,确定了最佳培养基组成为:葡萄糖30 g/L,蛋白胨7.5 g/L,酵母粉10 g/L,磷酸氢二钠7.5 g/L,柠檬酸0.5 g/L,pH 5.0,30℃。采用优化的培养基制备细菌纤维素,应用傅立叶红外光谱、热重分析和扫描电子显微镜对其结构和性能进行表征。红外光谱测试显示含有纤维素的特征吸收峰。热重分析结果表明细菌纤维素最大失重温度为319.06℃,热稳定性较好。扫描电镜观察到细菌纤维素的纳米网状结构。
|
| 关 键 词： | 木醋杆菌 培养基优化 |
Medium Optimization of Acetobacter xylinum HN001 |
| |
| DU Jing-jing,CAO Xian-ying,LIN Qiang,CHEN Sheng-jie,HU Ling-jun. Medium Optimization of Acetobacter xylinum HN001[J]. Journal of Cellulose Science and Technology, 2011, 19(1): 35-40 |
| |
| Authors: | DU Jing-jing CAO Xian-ying LIN Qiang CHEN Sheng-jie HU Ling-jun |
| |
| Affiliation: | DU Jing-jing1,2,CAO Xian-ying1,LIN Qiang1,CHEN Sheng-jie1,HU Ling-jun1,2(1.Key Laboratory of Ministry of Education for Application Technology of Chemical Materials in Hainan Superior Resources,2.College of Materials and Chemical Engineering,Hainan University,Haikou 570228,China) |
| |
| Abstract: | The medium optimization of Acetobacter xylinun HN001 was obtained by orthogonal experimental design.The culture media of Acetobacter xylinun HN001 was optimized as：Glucose 30 g/L,peptone 7.5 g/L,yeast extract 10 g/L,Na2HPO4 7.5 g/L,Citric acid 0.5 g/L,pH 5.0,30℃.In this study,bacterial cellulose was synthesized by incubation of Acetobacter xylinun HN001 in the modified culture medium.And they were characterized by FT-IR spectrum,scanning electron microscope（SEM） and thermo gravimetric analyzer.The characteristic adsorption peaks of bacterial cellulose（BC） were in FTIR spectra.The maximum weight loss temperature of BC was 319.06℃,thermal stability of BC was better.Nanostructure of BC was observed by SEM. |
| |
| Keywords: | Acetobacter xylinun HN001 medium optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
