An evaluation of extracts of five traditional medicinal plants from Iran on the inhibition of mushroom tyrosinase activity and scavenging of free radicals |
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Authors: | P Khazaeli R Goldoozian† F Sharififar‡ |
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Affiliation: | Department of Pharmaceutics, Research Center of Pharmaceutics, Kerman University of Medical Sciences;, Kerman University of Medical Sciences;and Department of Pharmacognosy, Research Center of Pharmaceutics, Kerman University of Medical Sciences, Kerman, Iran |
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Abstract: | This study aimed to evaluate the free radical scavenging and inhibition properties of five medicinal plants, including Quercus infectoria Olive., Terminalia chebula Retz. , Lavendula stoechas L., Mentha longifolia L., Rheum palmatum L., toward the activity of mushroom tyrosinase using l -tyrosine and l -3,4-dihydroxyphenylalanine ( l -DOPA) as the substrate. The methanol extracts of Q. infectoria and T. chebula showed strong radical scavenging effect in 2,2'-dipheny l -1-picrylhydrazyl (DPPH) assay (IC50 = 15.3 and 82.2 μg mL?1 respectively). These plants also showed inhibitory effects against the activity of mushroom tyrosinase in hydroxylation of l -tyrosine (85.9% and 82.2% inhibition, respectively). These two plants also inhibited the oxidation of l -DOPA similar to kojic acid as positive control (IC50 = 102.8 and 192.6 μg mL?1 respectively). In general Q. infectoria and T. chebula significantly inhibited tyrosinase activity and DPPH radical. Both activities were concentration-dependant but not in linear manner. It is needed to study the cytotoxicity of these plant extracts in pigment cell culture before further evaluation and moving to in vivo conditions. |
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Keywords: | DPPH pigmentation Quercus infectoria Terminalia chebula tyrosinase activity |
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