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Cell Wall-Denaturing Molecules for Plant Gene Modification
Authors:Risa Naka  Prof Kousuke Tsuchiya  Prof Keiji Numata
Affiliation:1. Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510 Japan

Contribution: ?Investigation (lead), Validation (equal), Visualization (lead), Writing - original draft (lead), Writing - review & editing (equal);2. Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510 Japan

Contribution: Conceptualization (lead), Supervision (equal), Validation (supporting), Visualization (supporting), Writing - original draft (supporting), Writing - review & editing (equal);3. Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510 Japan

Abstract:Zwitterionic molecules, such as zwitterionic liquids (ZILs) and polypeptides (ZIPs), are attracting attention for application in new methods that can be used to loosen tight cell wall networks in a biocompatible manner. These novel methods can enhance the cell wall permeability of nanocarriers and increase their transfection efficiency into targeted subcellular organelles in plants. Herein, we provide an overview of the recent progress and future perspectives of such molecules that function as boosters for cell wall-penetrating nanocarriers.
Keywords:expansin  gene technology  nanotechnology  plant cell wall  zwitterions  zwitterionic liquids  zwitterionic polypeptides
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