|
|||||
|
|
SNAP-Tag-Targeted MRI-Fluorescent Multimodal Probes |
|
| Authors: | Dr Ebaston Thankarajan Dr Shimrit Oz Dr Abed Saady Dr Kseniya Kulbitski Prof Mykhail O Kompanets Prof Moris S Eisen Dr Shai Berlin |
| Affiliation: | 1. Department of Neuroscience, Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, 3525422 Israel
Schulich Faculty of Chemistry, Technion – Israel Institute of Technology, Haifa, 3200003 Israel These authors contributed equally to this work. Contribution: Formal analysis (equal), ?Investigation (equal), Visualization (equal), Writing - original draft (equal), Writing - review & editing (equal);2. Department of Neuroscience, Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, 3525422 Israel These authors contributed equally to this work. Contribution: Data curation (equal), Formal analysis (equal), ?Investigation (equal), Methodology (equal), Project administration (equal), Visualization (equal), Writing - original draft (equal), Writing - review & editing (equal);3. Department of Neuroscience, Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, 3525422 Israel Schulich Faculty of Chemistry, Technion – Israel Institute of Technology, Haifa, 3200003 Israel Present address: School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ UK These authors contributed equally to this work. Contribution: Data curation (equal), Formal analysis (equal), ?Investigation (equal), Visualization (equal), Writing - review & editing (equal);4. Schulich Faculty of Chemistry, Technion – Israel Institute of Technology, Haifa, 3200003 Israel;5. L.M. Litvinenko Institute of Physico-Organic Chemistry and Coal Chemistry, National Academy of Sciences of Ukraine, Kyiv, 02660 Ukraine;6. Department of Neuroscience, Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, 3525422 Israel |
| Abstract: | Magnetic resonance imaging (MRI) is a powerful imaging modality, widely employed in research and clinical settings. However, MRI images suffer from low signals and a lack of target specificity. We aimed to develop a multimodal imaging probe to detect targeted cells by MRI and fluorescence microscopy. We synthesized a trifunctional imaging probe consisting of a SNAP-tag substrate for irreversible and specific labelling of cells, cyanine dyes for bright fluorescence, and a chelated GdIII molecule for enhancing MRI contrast. Our probes exhibit specific and efficient labelling of genetically defined cells (expressing SNAP-tag at their membrane), bright fluorescence and MRI signal. Our synthetic approach provides a versatile platform for the production of multimodal imaging probes, particularly for light microscopy and MRI. |
| Keywords: | contrast agents fluorescent probes imaging agents MRI SNAP-tag |