Factors affecting the susceptibility of Staphylococcus aureus CCRC 12657 to water soluble lactose chitosan derivative |
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| Affiliation: | 1. School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;2. School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, China;1. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil;2. Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil;3. Genome Investigation and Analysis Laboratory (GENIAL), Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil;1. Department of Phytochemistry & Chemical Engineering, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran;2. Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran;3. Department of Food Science, University of Massachusetts, Amherst, MA, 01003, USA;1. Graduate School of Eng., Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan;2. Graduate School of Sci., Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan;3. Diagnostics Technology Team, ITER Korea, National Fusion Research Institute, Deajeon, 305-333, Republic of Korea;4. National Institute for Fusion Science, 322-6 Oroshi-cho, Toki 509-5292, Japan;5. The Graduate University for Advanced Studies, 322-6 Oroshi-cho, Toki 509-5292, Japan;6. Fusion Research and Development Directorate, Japan Atomic Energy Agency, Tokai, Naka, Ibaraki 319-1195, Japan |
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| Abstract: | In this study, the susceptibility of Staphylococcus aureus CCRC 12657 to water-soluble lactose chitosan derivative as influenced by pH, temperature and cell age were investigated. Besides, the effect of this chitosan derivative on enterotoxin production and cell leakage of S. aureus CCRC 12657 was also examined.It was found that the susceptibility of S. aureus CCRC 12657 to lactose chitosan derivative varied with incubation temperature, pH and cell age. The lactose chitosan derivative exerted a higher antibacterial activity against S. aureus CCRC 12657 at 37°C than at 22 and 5°C. At pH 6.5–7.0, S. aureus CCRC 12657 was more susceptible to the chitosan derivative than at pH 6.0 or 7.5. Besides, cells of S. aureus CCRC 12657 in the late-exponential phase (12 h) were most susceptible to the chitosan derivative followed by cells in the stationary phase (24 h) and mid-exponential phase (6 h). Supernatant of cell suspension showed a marked increase in absorbance at 280 nm after the cells of the test organism were exposed to deionized water-containing lactose chitosan derivative. Furthermore, it was also noted that the lactose chitosan derivative not only caused the death of S. aureus CCRC 12657 but also inhibited enterotoxin production in Brain–Heart infusion broth. |
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