| 甲硝羟乙唑衍生物(CM)对离体乏氧L_(7712)翻胞的辐射增敏作用 |
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| 引用本文： | 杨立锡,郑秀龙.甲硝羟乙唑衍生物(CM)对离体乏氧L_(7712)翻胞的辐射增敏作用[J].辐射研究与辐射工艺学报,1987(1). |
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| 作者姓名： | 杨立锡 郑秀龙 |
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| 作者单位： | 第二军医大学
(杨立锡)，第二军医大学(郑秀龙) |
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| 摘 要： | 本文报道了用马血清代替小牛血清改进和建立了能用于检测L_(7712)细胞存活率的ADC法。并用此法研究证明了我们合成的甲硝羟乙唑衍生物(CM)对离体乏氧L_(7712)细胞有明显的辐射增敏作用,其SER值为2左右,较原化合物灭滴灵的SER(1.2)约提高67％。CM对缺氧细胞存活率的抑制作用比有氧下强。与前文报道的CM对荷瘤小鼠S_(180)缺氧下照射有辐射增敏作用及对鼠乏氧S_(180)生长有抑制作用的结果一致。作者对台盼蓝染色法和ADC法检测细胞存活率作了比较。
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| 关 键 词： | CM L_(7712)细胞 扩散盒 存活曲线 SER |
RADIOSENSITIZATION OF HYPOXIC L_(7712) CELLS BY METRONIDAZOLE DERIVATIVE (CM) |
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| Yang Lixi,Zheng Xiulong.RADIOSENSITIZATION OF HYPOXIC L_(7712) CELLS BY METRONIDAZOLE DERIVATIVE (CM)[J].Journal of Radiation Research and Radiation Processing,1987(1). |
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| Authors: | Yang Lixi Zheng Xiulong |
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| Affiliation: | Yang Lixi;Zheng Xiulong (S. L. Cheng) Second Military Medical College (Shanghai) |
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| Abstract: | The modified ADC method which can be used in measuring the sur-vival fraction of L_(7712) cells was established using horse serum instead of calf serum.It demonstrated the obvious radiosensitizing effect of metronidazole derivative (CM)on in vitro bypoxic L_(7712) cells. Its SER was about 2, and was about 67 percent hig-her than SER (1 .2) of the original compound, metronidazole. CM was found to bestronger under hypoxic condition than under aerated condition in the inbibition of cellsurvival fraction. It was consistent with the results of the preceeding paper that CMshowed a strong ability to sensitize sarcoma 180 to radiation and to inhibit the growthof sarcoma 180 under hypoxic condition. Using trypan blue staining method and theADC method to observe cell survival were compared. |
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| Keywords: | CM L_(7712) cells diffusion chamber survival curve SER |
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