Mutation breeding of nuclease p1 production in <Emphasis Type="Italic">Penicillium citrinum</Emphasis> by low-energy ion beam implantation |
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Authors: | Qinting He Nan Li Xiaochun Chen Qi Ye Jianxin Bai Jian Xiong Hanjie Ying |
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Affiliation: | (1) State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009 Nanjing, People’s Republic of China; |
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Abstract: | Nuclease p1 is an important enzyme in the nucleotide industry that is used to hydrolyze nucleic acid into nucleotides. To
improve enzyme activity, Penicillium citrinum, a nuclease p1 producing strain, was mutated by low-energy Nitrogen ion beam implantation at an energy level of 15 keV and
a dose ranging from 1×1015–1×1016 ions/cm2. The mutant strain designated as N409 was obtained with a high yield of nuclease p1. The activity of nuclease p1 was 421
U/mL from the mutant strain N409, which was increased by 86% compared with the control. The fermentation kinetics of nuclease
p1 by the mutant strain N409 was studied in a 30 L external airlifting bioreactor. A model was proposed using the logistic
equation for microbial growth, the Luedeking-Piret equation for product formation and a Luedeking-Piret-like equation for
substrate uptake. The results predicted from the model were in good agreement with the experimental observations. |
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