| 反相高效液相色谱法测定维生素B_1的含量 |
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| 引用本文： | 陈向明. 反相高效液相色谱法测定维生素B_1的含量[J]. 福建分析测试, 2012, 0(1): 50-51 |
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| 作者姓名： | 陈向明 |
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| 作者单位： | 滨州医学院药学院,山东烟台,264003 |
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| 摘 要： | 建立了测定维生素B1含量的高效液相色谱(HPLC)方法。方法采用ultimate C18柱(300×4.6mm,5μm)色谱柱,流动相为0.2%磷酸氢二钾水溶液(含5%甲醇,用磷酸调pH 2.6),检测波长245nm。维生素B1在0.002～2.0mg/mL范围内与峰面积呈现良好的线性关系(r=0.9968)。所建立的方法简单、准确、重现性好,可用于制剂中维生素B1含量的测定。
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| 关 键 词： | 维生素B1 高效液相色谱法 含量测定 |
Determination of Vitamin B1 Content by High Performance Liquid Chromatography |
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| Chen Xiang-ming. Determination of Vitamin B1 Content by High Performance Liquid Chromatography[J]. Fujian Anal Ysis & Testing, 2012, 0(1): 50-51 |
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| Authors: | Chen Xiang-ming |
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| Affiliation: | Chen Xiang-ming (College of Pharmacy, Binzhou Medical University, Yantai, Shandong 264003, China) |
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| Abstract: | A method for determination of vitamin B1 in Phamaey by high performance liquid chromatography was developed.Samples were separated on a ultimate C,8 column （300 × 4.6mm, 5 μm） with 5% methanol water solution as mobile phase, and the flow rate was 1.0mL/min. The detection wavelength was set at 245nm. The calibration curve of vitamin B1 showed good linearity in the range of 0.002- 2.0mg/mL, the correlation coefficients （R） was over 0.996. The method was simply, accurate and showed good repeatability. |
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| Keywords: | Vitamin B1 HPLC Content deferminate |
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