| RRLC测定发酵液中达托霉素的含量 |
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| 引用本文： | 周剑,谢颖,黄建峰,郑卫,方东升. RRLC测定发酵液中达托霉素的含量[J]. 福建分析测试, 2012, 0(1): 46-49 |
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| 作者姓名： | 周剑 谢颖 黄建峰 郑卫 方东升 |
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| 作者单位： | 福建省微生物研究所。福建省新药（微生物）筛选重点实验室,福建福州,350007 |
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| 基金项目： | 福建省自然科学基金资助（项目编号2011J01094） |
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| 摘 要： | 建立可快速准确测定玫瑰孢链霉菌发酵液中达托霉素含量的RRLC方法。采用高分离度快速液相色谱(RRLC)Agilent 1200 series,色谱柱:Aglient XDB C18,1.8u,4.6*50mm。流动相:0.1%三氟乙酸水溶液:乙腈=64:36,检测波长为214 nm,柱温为30℃。结果:达托霉素浓度范围在25～500ug/ml线性良好,相关系数为0.9989;测得发酵液样品的平均回收率为101.7%,RSD为0.63%。结论:方法准确可靠,可以用于达托霉素发酵过程的含量测定。
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| 关 键 词： | 达托霉素 发酵液 高分离度快速液相色谱(RRLC) |
RRLC Determination of Daptomycin in Fermentation Broth |
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| Zhou jian,Xie ying,Huang jian feng,Zheng wei,Fang dong sheng. RRLC Determination of Daptomycin in Fermentation Broth[J]. Fujian Anal Ysis & Testing, 2012, 0(1): 46-49 |
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| Authors: | Zhou jian Xie ying Huang jian feng Zheng wei Fang dong sheng |
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| Affiliation: | ( Fujiang Institute of Microbiology, Fujian Key Laboratory of screening for Novel Microbial Products, Fuzhou, 350007, China) |
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| Abstract: | Object：Develop a RRLC method for the determination of daptomycin in fermentation broth of Streptomycesroseosporus. Methods： Using RRLC Agilent 1200 series, Aglient XDB C18 （1.8u, 4.6＊50mm）, the mobile phase was 0. 1% Trifluoroacetic acid （TFA） ：acetonitrile=64：36 at a flow rate of 1.0mL.min-land detection wavelength was 214 nm, column temperature was 30 ℃. Results ： The calibration curve was linear over the range of 25-500ug/ml with the correlation 0.9989 （n=7）. The average recovery of daptomycin was 101.7% （RSD=0.63 % ）. Conclusion： The method is accurate, simple with good reproducibility, which is suitable for the determination of daptomycin in fermentation broth. |
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| Keywords: | daptomycin fermentation broth RRLC |
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