| 循环伏安法研究Cu^2＋与谷胱甘肽的配位作用及其分析应用 |
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| 引用本文： | 朱亚玲,张玉龙,崔胜云. 循环伏安法研究Cu^2＋与谷胱甘肽的配位作用及其分析应用[J]. 延边大学学报(自然科学版), 2009, 35(4): 345-349 |
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| 作者姓名： | 朱亚玲 张玉龙 崔胜云 |
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| 作者单位： | 延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002 |
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| 摘 要： | 通过Cu^2＋与谷胱甘肽（GSH）的配位作用,在过量Cu^2＋溶液中,利用循环伏安法测定剩余铜的阳极溶出峰电流的方法,间接测定了GSH的含量.实验表明,铜的氧化峰电流随溶液中GSH浓度的增加呈线性降低.用该方法测定7次的相对标准偏差（RSD）为0.513 2%,检测限达5.6×10^-4mmol/L.利用该方法测定针剂中的GSH含量,并与光度法的测定结果相比较,两者结果基本吻合.本研究提供了一种简便、可靠、快速地测定基体成分不复杂样品中GSH含量的分析方法.
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| 关 键 词： | 谷胱甘肽 循环伏安法 铜 电化学分析 |
Electrochemical Determination of Glutathione Using Complexation between Glutathione and Copper Ion |
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| ZHU Ya-ling,ZHANG Yu-long,CUI Sheng-yun. Electrochemical Determination of Glutathione Using Complexation between Glutathione and Copper Ion[J]. Journal of Yanbian University (Natural Science), 2009, 35(4): 345-349 |
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| Authors: | ZHU Ya-ling ZHANG Yu-long CUI Sheng-yun |
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| Affiliation: | ( Key Laboratory of Organism Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji 133002, China ) |
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| Abstract: | We discribe a indirect determination method of glutathione（GSH） using cyclic voltammetric determi nation according to the eomplexation interaction between copper ion and GSH. In the extra copper ion solutions, anodic stripping currents of copper is linearly decreased as the GSH concentration increased. The method is reproducible and sensitive with RSD 0. 513 2% （7 paralel determination） and detection limit 5. 6 × 10 ^-4 mmol/L. Satisfactory results were obtained by the determination of GSH in the injection sample and the results is correspondant with the result determined by UV-Vis spectrometric methods. |
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| Keywords: | glutathione cyclic voltammetry copper electroanalysis |
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