| 汞(Ⅱ)与明胶蛋白质相互作用的荧光光谱研究 |
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| 引用本文： | 唐世华.汞(Ⅱ)与明胶蛋白质相互作用的荧光光谱研究[J].明胶科学与技术,2006,26(2):69-73. |
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| 作者姓名： | 唐世华 |
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| 作者单位： | 广西民族大学化学与生态工程学院,广西,530006 |
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| 基金项目： | 广西民族大学校科研和教改项目 |
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| 摘 要： | 利用荧光光谱技术,研究了pH为7.30时Hg2+离子与明胶的相互作用,用不同的公式对实验结果进行对比处理,计算了结合常数(K)和结合位点数(n)。推测了Hg2+离子对明胶荧光的猝灭机制为Hg2+离子浓度较低时主要是动态猝灭,浓度较高时表现为静态猝灭。
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| 关 键 词： | 明胶 汞(Ⅱ)离子 荧光光谱 猝灭机制 |
Study of Interaction between Mercury(Ⅱ) and Gelatin Using Fluorescence Spectroscopy |
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| Authors: | Tang Shi-hua |
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| Affiliation: | College of Chemistry and Ecological Engineering, Caungxi University for Nationalities, Nanning 530006, Caungxi, P. R. China |
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| Abstract: | Interaction of gelatin with Hg~(2 ) ion was investigated by using the fluorescence.The binding constant(%K%) and number of binding sites(%n%) were determined.A quenching mechanism for fluorescence between gelatin and Hg~(2 ) ion was introduced.The dynamic quenching occured in lower concentration and the static quenching occured in higher concentration of Hg~(2 ) ion. |
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| Keywords: | gelatin mercury(Ⅱ) fluorescence spectroscopy quenching mechanism |
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