ESTIMATION OF DAILY INTAKE OF CHLOROFORM BY JAPANESE HOUSEWIVES |
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Authors: | MASATAKE TOYODA K KOBAYASHI AKIO MITSURA YUKIO SAITO KEIICHI UNO YOSHIO SAKABE |
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Affiliation: | Division of Foods National Institute of Hygienic Sciences 18-1, Kamiyoga, 1-chome, Setagaya-ku Tokyo 158, Japan;Aichi Prefectural Institute of Public Health 6-7, Nagare, Tsuji-machi, Kita-ku Nagoya 462, Japan;Nagoya City Health Research Institute 11-1, Hagiyama-cho, Mizuho-ku Nagoya 460, Japan |
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Abstract: | Duplicate portions of daily meals consumed by 21 housewives were collected for 3 consecutive days at Nagoya-city and Asuke-cho in Japan. The 24 h duplicate meals were analyzed for chloroform. Thirty grams of samples, 170 mL of water, 1 mL of phosphoric acid, and 0.5 g of ascorbic acid were mixed in a distillation flask which was heated under nitrogen gas flow. The purged chloroform was trapped in n-pentane cooled at 0° C. The pentane solution was analyzed to by electron capture gas chromatography. Confirmation of chloroform was carried out by GC-MS. The concentration of chloroform in each of the 24-h duplicate meals were in the range of 1.7 to 45.8 ppb and the average concentration was 13.7± 9.6 ppb. Daily dietary intake of chloroform by housewives were in the range of 7.5 to 51.9 μg and the mean intake was 26.4 ± 11.8 μg. |
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