气相色谱法测定食用油中BHA和BHT残留量的对比研究

目的建立气相色谱法测定食用油中叔丁基羟基茴香醚(butyl hydroxyanisole,BHA)和二丁基羟基甲苯(butylated hydroxytoluene,BHT)的残留量。方法使用无水乙醇萃取样品,经中性氧化铝吸附杂质,HP-5毛细管柱分离,使用气相色谱仪定量分析BHA和BHT在食用油中的残留量。同时,与GB/T 5009.30中的方法进行对比,考察了本方法的线性、重现性、回收率和精密度。结果本方法中BHA和BHT的线性范围分别为2.02~40.48 ng和2.07~41.36 ng,标准曲线方程分别为Y=16.61X+10.03(r=0.9996)和Y=20.14X+16.56(r=0.9997);BHA和BHT的检出限分别为92.4 ng/kg和108.6 ng/kg,加标回收率分别为98.94%(相对标准偏差为0.33%)、98.69%(相对标准偏差为0.34%)。结论本实验建立的方法灵敏度高、定量准确,和国标法相比,具有简单实用的特点,适合食用油中BHA和BHT残留量的检测。

Comparative study on the determination of residues of BHA and BHT in edible oil by gas chromatography

Objective To establish a method for the determination of butyl hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) in edible oil by gas chromatography (GC). Methods The BHA and BHT were extracted from samples with absolute alcohol, the analytes were separated on HP-5 column after adsorbing the impurities by neutral alumina and detected by GC. The study investigated the linear, repeatability, recovery and precision of this method, and the results were compared with GB/T 5009.30 method. Results The linear ranges of BHA and BHT in edible oil were 2.02~40.48 ng and 2.07~41.36 ng, the equations were Y=16.61X+10.03 (r=0.9996) and Y=20.14X+16.56 (r=0.9997), the limits of determination were 92.4 ng/kg and 108.6 ng/kg, the recoveries were 98.94% (relative standard deviation was 0.33%) and 98.69% (relative standard deviation was 0.34%), respectively. Conclusion This method is highly sensitive with good selectivity and accurate quantification, which is suitable for the determination of BHA and BHT residues in edible oil.