Enzymatic enrichment of arachidonic acid from Mortierella single-cell oil |
| |
Authors: | Yuji Shimada Akio Sugihara Yumi Minamigawa Kenichi Higashiyama Kengo Akimoto Shigeaki Fujikawa Sadao Komemushi Yoshio Tominaga |
| |
Affiliation: | (1) Osaka Municipal Technical Research Institute, 1-6-50 Morinomiya, Joto-ku, 536-8553 Osaka, Japan;(2) Department of Agricultural Chemistry, School of Agriculture, Kinki University, 631-8505 Nara, Japan;(3) Institute for Fundamental Research, Suntory Ltd., 618-0001 Osaka, Japan |
| |
Abstract: | An attempt was made to enrich arachidonic acid (AA) from Mortierella single-cell oil, which had an AA content of 25%. The first step involved the hydrolysis of the oil with Pseudomonas sp. lipase. A mixture of 2.5 g oil, 2.5 g water, and 4000 units (U) Pseudomonas lipase was incubated at 40°C for 40 h with stirring at 500 rpm. The hydrolysis was 90% complete after 40 h, and the resulting
free fatty acids (FFA) were extracted with n-hexane (AA content, 25%; recovery of AA, 91%). The second step involved the selective esterification of the fatty acids with
lauryl alcohol and Candida rugosa lipase. A mixture of 3.5 g fatty acids/lauryl alcohol (1:1, mol/mol), 1.5 g water, and 1000 U Candida lipase was incubated at 30°C for 16 h with stirring at 500 rpm. Under these conditions, 55% of the fatty acids were esterified,
and the AA content in the FFA fraction was raised to 51% with a 92% yield. The long-chain saturated fatty acids in the FFA
fraction were eliminated as urea adducts. This procedure raised the AA content to 63%. To further elevate the AA content,
the fatty acids were esterified again in the same manner with Candida lipase. The repeated esterification raised the AA content to 75% with a recovery of 71% of its initial content. |
| |
Keywords: | Arachidonic acid enrichment hydrolysis lauryl alcohol Pseudomonas sp lipase Rhizopus delemar lipase selective esterification urea adduct |
本文献已被 SpringerLink 等数据库收录! |
|