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| 腐植酸诱导人早幼粒白血病HL-60细胞凋亡 | |
| 引用本文： | Hsin-Ling Yang,You-Cheng Hseu,Yi-Ting Hseu,Fung-Jou Lu,Elong Lin,Jim-Shoung Lai,王晶晶,李宝才,李鹏,何静,吴远双. 腐植酸诱导人早幼粒白血病HL-60细胞凋亡[J]. 腐植酸, 2011, 0(3): 33-40 |
| 作者姓名： | Hsin-Ling Yang You-Cheng Hseu Yi-Ting Hseu Fung-Jou Lu Elong Lin Jim-Shoung Lai 王晶晶 李宝才 李鹏 何静 吴远双 |
| 作者单位： | 1. 台湾台中市中国医药大学营养协会,台中,000400 2. 台湾台中市中泰保健科技协会食品科学研究室,台中,000400 3. 台湾台中市中山医学大学应用化学研究室,台中,000400 4. 台湾台中市中国医药大学环境卫生毕业生协会,台中,000400 5. 昆明理工大学生命科学与技术学院,昆明,650224 |
| 摘 要： | 试验采用人早幼粒白血病HL-60细胞,主要通过细胞活性丧失,染色质凝聚、核小体间DNA断裂等凋亡特征,研究了腐植酸诱导细胞凋亡的效应,观察了不同时间不同剂量(50～400 μg/mL)腐植酸对细胞凋亡的影响.腐植酸诱导HL-60细胞凋亡主要与线粒体内细胞色素c的释放有关,并证明伴随着Caspase-3(天冬氨酸特异性半... |
| 关 键 词： | 腐植酸 HL-60细胞 细胞凋亡 |
Humic Acid Induces Apoptosis in Human Premyelocytic Leukemia HL-60 Cells |
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| Hsin-Ling Yang,You-Cheng Hseu,Yi-Ting Hseu,Fung-Jou Lu,Elong Lin,Jim-Shoung Lai,Wang Jingiing,Li Baocai,Li Peng,He Jing,Wu Yuanshuang. Humic Acid Induces Apoptosis in Human Premyelocytic Leukemia HL-60 Cells[J]. , 2011, 0(3): 33-40 | |
| Authors: | Hsin-Ling Yang You-Cheng Hseu Yi-Ting Hseu Fung-Jou Lu Elong Lin Jim-Shoung Lai Wang Jingiing Li Baocai Li Peng He Jing Wu Yuanshuang |
| Affiliation: | Hsin-Ling Yang1,You-Cheng Hseu2,Yi-Ting Hseu1,Fung-Jou Lu3,Elong Lin2,Jim-Shoung Lai4 write Wang Jingjing5,Li Baocai5*,Li Peng5,He Jing5,Wu Yuanshuang5 translate(1 Institute of Nutrition,China Medical University,Taichung,Taiwan,000400 2 Department of Food Science,Chungtai Institute of Health Sciences and Technology,000400 3 Department of Applied Chemistry,Chun Shan Medical University,000400 4 Graduate Institution of Environmental Health,Taiwa... |
| Abstract: | It has been shown that humic acid,a phenolic polymer,exhibits pro-oxidant and cytotoxic effects.In this study,HA induction of apoptosis was studied using cultured human premyelocytic leukemia HL-60 cells.Treatment at a range of HA concentrations（50～400 μg/mL） resulted in dose-and time-dependent sequences of events marked by apoptosis,as demonstrated through by apoptotic features such as loss of cell viability,chromatin condensation,and internucleosomal DNA fragmentation.This HA-induced apoptosis in the HL-60 cells was mainly associated with the release of cytochromec from the mitochondria.Furthermore,apoptosis in the HL-60 cells was accompanied by the activation of Caspase-3 and the specific proteolytic cleavage of poly（ADP-ribose） polymerase（PARP）,a major component in the apoptotic cell death mechanism.Although the HA-induced apoptosis was associated with Bax protein levels,negligible Bcl-2 reduction was observed.Analysis of the data reported herein reveals that HA exerts antiproliferative action and growth inhibition on HL-60 cells through induction of apoptosis,which may have anticancer properties potentially useful for the development of new drug products. |
| Keywords: | humic acid HL-60 cells apootosis |
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