| Vero细胞和流感病毒在无血清条件下的微载体培养 |
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| 引用本文： | 何春艳,陈列胜,吴书军,唐丽丽. Vero细胞和流感病毒在无血清条件下的微载体培养[J]. 中国生物制品学杂志, 2007, 20(2): 125-128 |
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| 作者姓名： | 何春艳 陈列胜 吴书军 唐丽丽 |
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| 作者单位： | 上海生物制品研究所研发部 上海200052 |
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| 摘 要： | 目的探索Vero细胞和流感病毒在无血清条件下的微载体培养条件。方法在搅拌瓶中,选择合适的微载体,在无血清条件下培养Vero细胞和流感病毒。观察Vero细胞的生长状况,并检测流感病毒的血凝滴度。结果Vero细胞在Cytodex 3上生长最好,每个微载体上接种10个细胞为最佳接种浓度,以MOI=0.005和0.010 CCID50/细胞接种H1N1流感病毒后,在72h时,培养上清病毒血凝滴度达到最高。结论无血清条件下,Vero细胞在Cytodex 3上生长良好,流感病毒能在Vero细胞上成功培养。
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| 关 键 词： | 无血清培养 微载体 Vero细胞 流感病毒 |
| 文章编号： | 1004-5503（2007）02-125-04 |
| 收稿时间： | 2006-03-28 |
| 修稿时间： | 2006-03-28 |
Serum-free Culture of Vero Cells and Influenza Virus on Microcarrier |
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| HE Chun-yan, CHEN Lie-sheng, WU Shu-jun, et al. Serum-free Culture of Vero Cells and Influenza Virus on Microcarrier[J]. Chinese Journal of Bilogicals, 2007, 20(2): 125-128 |
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| Authors: | HE Chun-yan CHEN Lie-sheng WU Shu-jun et al |
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| Affiliation: | Shanghai Institute of Biological Products, Shanghai 200052, China |
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| Abstract: | Objective To explore the condition for serum-free culture of Vero cells and influenza virus on microcarrier.Methods Culture Vero cells and influenza virus in serum-free medium by using microcarriers in stirring flask.Observe the growth and adsorption of Vero cells and determine the hemagglutination titer of virus.Results Vero cells grew well on Cytodex 3 microcarrier.The optimal seeding density of Vero cells was 10 cells per bead.The hemagglutination titer of virus reached the maximum 72 h after inoculateion at a MOI of 0.005 or 0.010 CCID_(50)/cell.Conclusion In the absence of serum,Vero cells grew well on Cytodex microcarrier,and influenza virus was successfully cultured in the cells. |
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| Keywords: | Serum-free culture Microcarrier Vero cells Influenza virus |
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