| 应用异型双功能试剂连接制备乳胶妊娠诊断制剂 |
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| 引用本文： | 张洪雁,凌晓阳,董春娥,周连福,刘素文,孙石. 应用异型双功能试剂连接制备乳胶妊娠诊断制剂[J]. 中国生物制品学杂志, 2000, 13(1): 36-37 |
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| 作者姓名： | 张洪雁 凌晓阳 董春娥 周连福 刘素文 孙石 |
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| 作者单位： | [1]吉林省计划生育研究所,长春 [2]吉林省医药情报研究所,长春 |
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| 基金项目： | 本课题为吉林省科委科学技术研究基金 |
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| 摘 要： | 目的研制稳定性特异性更好的妊娠诊断制剂。方法应用抗ＨＣＧ－β亚基Ｃ末端多肽抗体，以双 功能试剂连接到羧化苯乙烯乳胶上。结果制成的妊娠诊断试剂较目前通用的ＨＣＧ乳胶试剂灵敏度高，为１．５ＩＵ／ ｍｌ。稳定性好，在室温放置３个月，其灵敏度未下降。用本试剂检测１６０份妊娠尿和１５０份正常尿，结果准确率为 ９５％。结论本试剂所用方法优于通常应用的妊娠诊断方法。
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| 关 键 词： | 双功能试剂 妊娠诊断 单克隆抗体 |
| 修稿时间： | 1999-01-12 |
Preparation of Latex Diagnostic Kit for Pregnancy by Linking of Allotypic Bifunctional Reagents |
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| Zhang Hongyan,Ling Xiaoyang,Dong Chun''''e. Preparation of Latex Diagnostic Kit for Pregnancy by Linking of Allotypic Bifunctional Reagents[J]. Chinese Journal of Bilogicals, 2000, 13(1): 36-37 |
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| Authors: | Zhang Hongyan Ling Xiaoyang Dong Chun''''e |
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| Affiliation: | Zhang Hongyan,Ling Xiaoyang,Dong Chun'e(Jilin Institute of Family Planning, Changchun 130041) |
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| Abstract: | Objective To prepare a stable and specific diagnostic kit for pregnancy Methods A diagnos- tic kit for pregnancy was prepared by linking of C - terminal polypeptide antibody of anti - HCG - subunit to car- boxyphenylethene latex with bifunctional reagents .Results Latex agglutination test showed that the sensitivity of the kit was 1.5 IU/ml. After being stored at room temperature for 3 months,no decrease of the sensitivity was ob- served. One huned sixty urine specimens from pregnant women and 150 normal urine were detected by the kit. The result showed that the accuracy rate of the kit was 95% .Conclusion The kit is superior to routine kit. |
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| Keywords: | Bifunctional reagent Diagnosis of pregnacy McAb |
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