| 一种快速定性和定量检测发酵乳中L.fermentum的方法 |
| |
| 引用本文： | 包秋华,陈永福,张家超,孙志宏,包艳,张和平. 一种快速定性和定量检测发酵乳中L.fermentum的方法[J]. 中国乳品工业, 2011, 39(2) |
| |
| 作者姓名： | 包秋华 陈永福 张家超 孙志宏 包艳 张和平 |
| |
| 作者单位： | 内蒙古农业大学,乳品生物技术与工程教育部重点实验室,呼和浩特,010018 |
| |
| 基金项目： | 973计划前期研究专项，教育部创新团队发展计划，国家自然科学基金项目 |
| |
| 摘 要： | 通过比对发酵乳杆菌(L.fermentum)与其他乳酸菌的16S rRNA基因序列的异同,设计出L.fermentum的种属特异性引物,应用此引物进行种属特异性PCR和实时荧光定量PCR反应,通过琼脂糖凝胶电泳图谱和实时荧光定量PCR图谱分析,建立一种快速检测发酵乳中L.fermentum定性和定量测定方法.
|
| 关 键 词： | 乳酸菌 发酵乳杆菌 实时荧光定量PCR |
A rapid qualitative and quantitative detection method of L.fermentum in the fermented milk |
| |
| BAO Qiu-hua,CHEN Yong-fu,ZHANG Jia-chao,SUN Zhi-hong,BAO Yan,ZHANG He-ping. A rapid qualitative and quantitative detection method of L.fermentum in the fermented milk[J]. China Dairy Industry, 2011, 39(2) |
| |
| Authors: | BAO Qiu-hua CHEN Yong-fu ZHANG Jia-chao SUN Zhi-hong BAO Yan ZHANG He-ping |
| |
| Affiliation: | BAO Qiu-hua,CHEN Yong-fu,ZHANG Jia-chao,SUN Zhi-hong,BAO Yan,ZHANG He-ping(Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education ministry Inner Mongolia Agricultural University,Hohhot Inner Mongolia 010018,China) |
| |
| Abstract: | The differences in 16S rRNA gene region between L.fermentum and other Lactic acid bacteria were compared.The species specificity primer of L.fermentum was designed and used to carry out species-specific PCR assay and fluorescent quantitative real-time PCR.Through analyzing the electrophoretic pattern and the curve of RT-PCR,a rapid qualitative and quantitative method for the detection of L.fermentum in the fermented milk was established. |
| |
| Keywords: | PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
