In vivo lipolysis induced by heparin injection or fasting: a proton MR spectroscopy analysis of human plasma |
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Authors: | Pascale Silvestre Jean Vion-Dury Mostafa Kriat Patrick Viout Louis Cara Jean Pierre Ardissone Bernard Vialettes Patrick J Cozzone |
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Affiliation: | (1) Service de Nutrition, Maladies Métaboliques et Endocrinologie, CHU Timone, Marseille, France;(2) Faculté de Médecine, Centre de Résonance Magnétique Biologique et Médicale, Marseille, France;(3) Unité 130, INSERM, Marseille, France |
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Abstract: | The impact of induced lipolysis on the composition of plasma lipids is analyzed by proton magnetic resonance spectroscopy (MRS) in humans. The variations of the methylene and methyl resonances from lipids in lipoproteins are studied under two sets of lipolytic conditions: acute endovascular lipolysis induced by an intravenous injection of heparin and subacute lipolysis induced by short fasting. During acute lipolysis, the degradation of the very low density lipoproteins structures is well correlated to the modifications observed in the areas of CH2 and CH3 MRS signals. The comparison of regular spectra, spectra with water signal suppression, and spectra recorded with a spin-echo sequence provides information on the behavior of the different parts of the lipoproteins, that is, the neutral core, little affected by heparine-induced lipoprotein lipase (LPL) activation, and the surface layer supplying substrates to LPL. During 48 h of fasting, only limited modifications occur on the MR spectra, and lipolysis cannot be documented in details.Address for correspondence: CRMBM-CNRS, Faculté de Médecine, 27, bd Jean Moulin, 13005 Marseille, France. |
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Keywords: | high-resolution proton MR spectroscopy lipolysis plasma heparin lipoprotein lipase |
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