| 荧光假单胞杆菌磷脂酶B界面催化分子机制初探 |
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| 引用本文： | 赵依威,姜芳燕,李春,戴大章. 荧光假单胞杆菌磷脂酶B界面催化分子机制初探[J]. 化工学报, 2020, 71(9): 4255-4259. DOI: 10.11949/0438-1157.20200527 |
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| 作者姓名： | 赵依威 姜芳燕 李春 戴大章 |
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| 作者单位： | 北京理工大学化学与化工学院，北京 100081 |
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| 摘 要： | 基于荧光假单胞杆菌磷脂酶B (Pf-PLB)一级序列,分析了Pf-PLB的相关理化性质及其二级结构,并采用同源建模的方法对Pf-PLB的三级结构进行了预测;基于其预测结构,提出"掀盖进入,侧向离去"假说模型,初步探讨了Pf-PLB界面催化的可能机制;采用定点突变的方法对Pf-PLB的结构进行改造,结合突变体Pf-PLB的催化特性对所提出的机制模型进行了验证。
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| 关 键 词： | 磷脂酶B 结构预测 催化机制 |
| 收稿时间： | 2020-05-08 |
Interfacial catalytic mechanism of Pseudomonas fluorescens phospholipase B |
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| ZHAO Yiwei,JIANG Fangyan,LI Chun,DAI Dazhang. Interfacial catalytic mechanism of Pseudomonas fluorescens phospholipase B[J]. Journal of Chemical Industry and Engineering(China), 2020, 71(9): 4255-4259. DOI: 10.11949/0438-1157.20200527 |
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| Authors: | ZHAO Yiwei JIANG Fangyan LI Chun DAI Dazhang |
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| Affiliation: | School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China |
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| Abstract: | The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B（Pf-PLB） were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A “lid-in, side-out” hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB. |
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| Keywords: | phospholipase B structure prediction catalytic mechanism |
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