驯化的西湖底泥还原腐殖质影响因素研究

利用驯化的西湖底泥,以蒽醌-2,6-双磺酸盐（AQDS）作为腐殖质模式物,初步研究了温度、pH、不同类型氮源、不同种类金属离子等对底泥微生物还原腐殖质的影响.结果发现,在15～45 ℃下驯化底泥能还原腐殖质,最适反应温度为30～37 ℃；在反应初始pH为4～9的条件下可进行腐殖质还原,最适pH为7；不同类型氮源对微生物还原腐殖质的效果依次为NH4Cl＞CO(NH2)2＞NaNO3＞NaNO2；Mg2+、Mn2+促进了微生物还原腐殖质,而Zn2+、Ni2+、Cu2+部分地抑制了腐殖质还原,Hg2+则完全抑制腐殖质还原.

Influence factors of humus reduction by acclimated sediments of West Lake

By using acclimated sediments of West Lake, the effects of temperature, pH, different nitrogen sources, and different metal ions on humus microbial reduction were preliminarily analyzed with anthraquinone-2,6-disulfonate (AQDS) as humus model compound. Experimental results show that acclimated sediments can reduce humus during 15～45 ℃ and the optimum temperature is 30～37 ℃. Humus reduction was occurred at initiative pH 4～9, and the optimum pH is 7. The degree of humus reductive activities caused by different nitrogen sources is NH4Cl> CO(NH2)2> NaNO3> NaNO2. Mg2+、Mn2+ enhanced humus microbial reduction, but Zn2+、Ni2+、Cu2+ showed partial inhibition for humus reduction, and Hg2+ completely inhibited humus reduction.