Porous Poly(vinyl alcohol) Composite Membranes for Immobilization of Glucose Oxidase

Particle loaded porous poly(vinyl alcohol) composite membranes were selected for immobilization of glucose oxidase (GOx) for their hydrophilicity and unique interactions with amino functional groups. GOx was immobilized on the membranes by adsorption at pH values between 3.5 and 7.1. The highest adsorption loading was observed at pH 7.1 and the highest catalytic activity was observed at pH 5.1. Infrared studies showed that the highest ratio of amide I to amide II at pH 5.1 is obtained for GOx immobilized on membranes loaded with amine-functionalized micro-particles, suggesting that the conformational changes of GOx on these membranes yield to higher catalytic activity than in other supports.