牛肝菌胞外多糖发酵培养基的优化

在Plackett Burman设计实验结果基础上 ,采用响应曲面法对影响牛肝菌 (Boletussp .)ACCC 5 0 3 2 8发酵胞外多糖的培养基 5个关键组成成分酵母膏 (X1)、麦芽糖 (X2 )、(NH4 ) 2 SO4(X3)、FeSO4 (X4 )和CuSO4 ·5H2 O(X5)的最佳水平范围进行了研究和探讨。通过对二次多项回归方程求解得知 ,在上述自变量取值分别为 :酵母膏 1 4 .2 g/L ,麦芽糖 2 2 .2g/L ,(NH4 ) 2 SO4 2 .7g/L ,FeSO4 66.9mg/L ,CuSO4 1 0 1 .5 9μg/L时 ,胞外多糖最大预测值为 75 2 .0 79μg/mL(发酵醪 ) ,此预测可信度不仅被统计分析所验证 ,也被实践所证实

Optimization of Media for Exopolysaccharide Production by Boletus sp.ACCC 50328

Response surface methodology (RSM) based on a five-level five-factor central composite rotatable design of experiments was used to optimize the optimal levels of five important factors, namely yeast extract (X 1), maltose (X 2), ammonium sulfate (X 3), ferrous sulfate (X 4), copper sulfate pentahydrate (X 5). These five factors were chosen based on the results of our previous Plackett Burman design. Using the quadratic regression model, the optimum concentration levels for obtaining 752.079 microgram exopolysaccharide per milliliter were: 14.2 g/L yeast extract, 22.2 g/L maltose, 2.7 g/L ammonium sulfate, 66.9 mg/L ferrous sulfate and 101.59 μg/L copper sulfate pentahydrate. The predicted values were also verified by our experiment.