化学生物组合法合成米格列醇及其表征

以葡萄糖和乙醇胺为原料,催化加氢生成N-羟乙基葡糖胺,葡萄糖转化率为89%。氧化葡萄糖酸杆菌Gouv2007的静息细胞在通气下氧化N-羟乙基葡糖胺脱氢生成米格列醇的前体物质6-脱氧-6-羟乙基氨基-α-L-呋喃山梨糖,再催化加氢生成米格列醇,对从N-羟乙基葡糖胺生成米格列醇的底物转化率为77.3%。采用熔程测定、红外光谱分析、质谱分析对N-羟乙基葡糖胺和米格列醇进行了表征,结果表明:所合成的产物分别被鉴定为N-羟乙基葡糖胺和米格列醇。

Synthesis and characterization of miglitoi by combined biotechnological chemical synthesis method

Glucose and ethanolamines were catalytic hydrogenated to obtain N-(2-hydroxyethyl)-glucamine,with a transformation rate of 89%.N-(2-hydroxyethyl)-glucamine was dehydrogenated to 6-(2-droxyethyl)amino-6-deoxy-a-L-sorbofiuanose the key intermediate of miglitol by the resting cells of Gluconobater oxydans Gouv2007 through aeration.6-(2-Droxyethyl)amino-6-deoxy-a-L-sorbofiuanose was hydrogenated catalyticlly to miglitol.In the transformation of N-(2-hydroxyethyl)-glucamine to miglitol,the transformation rate wa...